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Genetic changes in flat type and polypoid type colo-rectal cancer in early stages

扁平型和息肉型结直肠癌早期基因改变

基本信息

批准号：
10671213
负责人：
KONISHI Fumio
金额：
$ 1.22万
依托单位：
Jichi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671213/
关键词：
flat type colorectal cancer polypoid type colorectal cancer microsatellite instability apoptosis p53 p53過剰発現 APC β-アテニン表面型大腸早期癌隆起型大腸早期癌形態発生 DNAreplication error 大腸多発癌

项目摘要

The present research was conducted to clarify the genetic changes in flat type early colorectal carcinomas and polypoid type early colorectal carcinomas. The percentage of Ki-ras point mutation was significatly smaller in flat type than in polypoid type lesions. In the right side of the colon, the percentage of microsatellite instability was higher in flat type than in polypoid type carcinomas in early stages. In more advanced carcinomas of the right side of the colon, the MSI positive lesions showed higher percentage of Ki-ras mutation. Therefore, in the right side of the colon, flat type carcinomas in early stages can be considered to be a candidate for the MSI positive advanced carcinoma. However, in the left side of the colon and rectum, such results were not demonstrated. In flat-type carcinoma apoptotic index (Tunnel Index) in the p53 protein overexpression group was significantly smaller than that in the p53 protein-negative group (P < 0.05). The Ki-67 labeling index/Tunnel ratio in the p53 protein overexpression group was significantly higher than that in the p53 protein-netative tour (P < 0.05). In rolvroid-type carcinoma, no significant difference in the ratio was shown between the p53 protein overexpression group and p53 protein-negative group. Therefore, p53-dependent apoptosis may contribute to the development of flat-type early colorectal carcinoma. In summery, it was considered that there would be different genetic changes involved in the two different types of colorectal carcinomas in early changes.

本研究旨在阐明扁平型早期结直肠癌和息肉型早期结直肠癌的基因变化。扁平型的Ki-ras点突变百分率明显低于息肉样型。在右侧结肠，早期扁平型癌的微卫星不稳定性高于息肉样癌。在右半结肠癌中，MSI阳性病变的Ki-ras突变率较高。因此，在右侧结肠，早期扁平型癌可被认为是MSI阳性晚期癌的候选对象。然而，在左侧的结肠和直肠，没有显示出这样的结果。在扁平型癌中，P53蛋白过表达组的细胞凋亡指数(Tunes Index)明显小于P53蛋白阴性组(P&lt；0.05)。P53蛋白过表达组Ki-67标记指数/隧道比值显著高于P53蛋白阴性组(P&lt；0.05)。在滚状型癌中，P53蛋白过表达组与P53蛋白阴性组之间的比值无显著差异。因此，P53依赖的细胞凋亡可能参与了扁平型早期结直肠癌的发生发展。综上所述，认为两种不同类型的结直肠癌在早期变化中所涉及的基因变化可能不同。