Measurement of cerebral inhibitory neuronal receptors with positron emission tomography in relation to neuronal injury.

用正电子发射断层扫描测量脑抑制性神经元受体与神经元损伤的关系。

• 批准号: 10671287
• 负责人: NARIAI Tadashi
• 金额: $ 2.05万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671287/
• 关键词: PET; Receptors; adenosine; benzodiazepine; cerebral ischemia; neurotrauma; cerebral blood flow; cats; ネコ; 脳血流

Eighteen adult cats were intubated and anesthetized with 1.5% isoflurane, and were held by plastic ear bars to obtain a reproducible head position. In thirteen cats, right MCA was exposed with transorbital approach and was ready to be occluded with a microvascular clip. CBF before and during occlusion of MCA, and that immediately after reperfusion was quantified with i.v. bolus injection of ィイD115ィエD1O labeled water (200MBq per cat) and a PET instrument for animal scan, SHR2000 (Hamamatsu photonics, FWHM=4mm). Adenosine A1 receptor binding potential, central benzodiazepine receptor binding potential and CMRglc was serially quantified with 11C-MPDX (200MBq), 11C-flumazenil (200MBq) 18F-FDG (100 MBq) respectively. All PET parameters were presented as the right (ischemic)/left (control) ratio. The data of ischemic cat was compared with that of normal cats (N=5). Degree of ischemic damage was evaluated by histological evaluation and/or by T2 weighted MR image with animal MRI system (Unity … More plus, Varian). Four cats did not survive the 1st day because of brain edema and were classified as most severe ischemic insult. Three cats survived and had the infarction both in cortex and striatum. The other six had infarcted lesion only in striatum. CBF during occlusion of MCA was significantly reduced in all 3 ischemic group than in normal group. However, no statistically significant difference was found among the ischemic groups. Right/Left ratio of CBF after reperfusion and that of FDG uptake did not show any statistically significant difference among all groups. Adenosine A1 receptor binding potential and benzodiazepine receptor binding potential were significantly reduced in group with severe ischemic insult than that with normal or milder insult. Of two receptors examined, reduced binding potential of adenosine A1 receptor was more sensitive to the degree of ischemic insult. In this study, the degree of decreased binding of adenosine A1 receptor after reperfusion and the degree of ischemic insult correlated well. PET imaging of cerebral adenosine receptor with C-11 MPDX can be a suitable in vivo imaging technique to evaluate the function of adenosine and adenosine receptor in relation to cerebral ischemia. Less

对18只成年猫进行插管，并用1.5%异氟烷麻醉，并用塑料耳棒固定，以获得可重现的头部位置。在13只猫中，通过经眶入路暴露右侧MCA，并准备用微血管夹闭塞。采用PET动物扫描仪SHR 2000（Hamamatsu photonics，FWHM= 4 mm），静脉推注200 MBq/猫的2000 MBq的D115-D1 O标记水，定量测定MCA阻断前、阻断中及再灌注后即刻的CBF。分别用11 C-MPDX（200 MBq）、11 C-flumazenil（200 MBq）、18F-FDG（100 MBq）对腺苷A1受体结合电位、中枢苯二氮卓受体结合电位和CMRglc进行连续定量。所有PET参数均表示为右侧（缺血）/左侧（对照）比值。将缺血猫的数据与正常猫（N=5）的数据进行比较。通过组织学评价和/或使用动物MRI系统（Unity）通过T2加权MR图像来评价缺血损伤程度 ...更多信息 加上，瓦里安）。4只猫因脑水肿在第1天未存活，并被归类为最严重的缺血性损伤。3只存活猫同时出现皮层和纹状体梗死。其余6例仅在纹状体有梗死灶。3个缺血组MCA闭塞时的CBF均较正常组显著降低。然而，在缺血组之间没有发现统计学显著差异。各组再灌注后CBF的右/左比值和FDG摄取的右/左比值无统计学差异。腺苷A1受体结合电位和苯二氮卓类受体结合电位在严重缺血组明显低于正常或轻度缺血组。两个受体检查，腺苷A1受体的结合电位降低更敏感的缺血损伤的程度。在这项研究中，再灌注后腺苷A1受体结合减少的程度与缺血损伤的程度相关。脑腺苷受体的C-11 MPDX PET显像是一种合适的在体显像技术，可用于评价腺苷和腺苷受体在脑缺血中的作用。少

项目成果

Ishiwata,K,et al.: "Metabolic analysis of[C11]flumazenil in human plasma : Assessment as the standardized value for quantitative PET studies." Ann Nucl Med 1998 ; 12(1) : 55-59.

Ishiwata,K,et al.：“人血浆中[C11]氟马西尼的代谢分析：作为定量 PET 研究的标准化值的评估。”

Noguchi,J,et al.: "Evaluation of carbon-11-labeled KF17837 : a potential CNS adenosine A2a receptor ligand." J Nucl Med 1998 ; 39(3) : 498-503.

Noguchi,J,et al.：“碳 11 标记的 KF17837 的评估：一种潜在的 CNS 腺苷 A2a 受体配体。”

成相直: "動物用PET-脳機能の研究 : 疾患モデルを用いて." PET通信1998 25 : 12-14.

Nariso Nao：“动物 PET - 脑功能研究：使用疾病模型。”PET Tsushin 1998 25：12-14。

Shimada Y, et al.: "Decrease in adenosine A1 receptor binding potential after reperfusion as a predictor of severe cerebral ischemic insult-PET multitracer study in cats' model of transient MCA occlusion."J Cereb Blood Flow Metab. 19. S349 (1999)

Shimada Y 等人：“再灌注后腺苷 A1 受体结合电位降低作为严重脑缺血损伤的预测因子 - 在猫短暂 MCA 闭塞模型中进行 PET 多示踪剂研究。”J Cereb Blood Flow Metab。

Jiang XB et al.: "Changes in local cerebral blood flow, glucose utilization, and mitochondrial function following traumatic brain injury in rats."Neurol Med Chir (Tokyo). 40(1). 16-29 (2000)

Jiang XB 等人：“大鼠脑外伤后局部脑血流、葡萄糖利用和线粒体功能的变化。”Neurol Med Chir（东京）。