Measurement of cerebral inhibitory neuronal receptors with positron emission tomography in relation to neuronal injury.

十八只成年猫被诱导和怪异，具有1.5%的异氟素，并由塑料耳棒支撑，以获得可再生的头部位置。在13只猫中，正确的MCA已经用一种转移方法暴露了，并且已经准备好用一个微血管剪辑封闭了。CBF before and during occlusion of MCA， and that immediately after reperfusion was quantified with i.v. bolus injection ofイD115イエD1O labeled water （200 MBq per cat） and a PET instrument for animal scan， SHR2000 （Hamamatsu photonics， FWHM= 4 mm）。腺苷A1受体结合潜能、中苯并二氮杂卓受体结合潜能和CMRglc已按11 C-MPDX（200 MBq）、11 C-氟马替尼（200 MBq） 18F-FDG （100 MBq）的比例进行了全面量化。所有PET参数均为right （ischemic）/left （control） ratio。与普通猫的数据（N=5）相比较。通过历史评估评估和/或通过动物MRI系统T2加权磁共振成像（Unity） ... More 还有， Varian）。四只猫在第一天没有存活下来，因为大脑的ED被分类为最严重的化学污染。三只猫幸存下来，并在Cortex和Striatum中发现了一种炎症。其他六只被感染的狮子只在楼梯上。CBF在所有3个形态学群体中明显减少了MCA的封闭性，低于正常群体。然而，没有统计上有意义的差异是在这一现象中发现的。再灌注后CBF的右/左比率，即FDG在所有群体中没有显示出任何统计上有意义的差异。腺苷A1受体结合潜能和苯并二氮杂卓受体结合潜能在一组具有严重化学性的程度高于正常或中等性的程度。对两个受体进行了审查，并减少了腺苷A1受体的结合潜力，使其对化学中毒的程度更加敏感。在这项研究中，对灌注后腺苷A1受体的降解绑定程度和化学污染相关井的程度进行了研究。带有C-11 MPDX的半胱氨酸受体的PET成像可以是一种适合的体内成像技术，以评估腺苷和腺苷受体在与半胱氨酸相关方面的功能。Less（低）

Eighteen adult cats were intubated and anesthetized with 1.5% isoflurane, and were held by plastic ear bars to obtain a reproducible head position. In thirteen cats, right MCA was exposed with transorbital approach and was ready to be occluded with a microvascular clip. CBF before and during occlusion of MCA, and that immediately after reperfusion was quantified with i.v. bolus injection of ィイD115ィエD1O labeled water (200MBq per cat) and a PET instrument for animal scan, SHR2000 (Hamamatsu photonics, FWHM=4mm). Adenosine A1 receptor binding potential, central benzodiazepine receptor binding potential and CMRglc was serially quantified with 11C-MPDX (200MBq), 11C-flumazenil (200MBq) 18F-FDG (100 MBq) respectively. All PET parameters were presented as the right (ischemic)/left (control) ratio. The data of ischemic cat was compared with that of normal cats (N=5). Degree of ischemic damage was evaluated by histological evaluation and/or by T2 weighted MR image with animal MRI system (Unity … More plus, Varian). Four cats did not survive the 1st day because of brain edema and were classified as most severe ischemic insult. Three cats survived and had the infarction both in cortex and striatum. The other six had infarcted lesion only in striatum. CBF during occlusion of MCA was significantly reduced in all 3 ischemic group than in normal group. However, no statistically significant difference was found among the ischemic groups. Right/Left ratio of CBF after reperfusion and that of FDG uptake did not show any statistically significant difference among all groups. Adenosine A1 receptor binding potential and benzodiazepine receptor binding potential were significantly reduced in group with severe ischemic insult than that with normal or milder insult. Of two receptors examined, reduced binding potential of adenosine A1 receptor was more sensitive to the degree of ischemic insult. In this study, the degree of decreased binding of adenosine A1 receptor after reperfusion and the degree of ischemic insult correlated well. PET imaging of cerebral adenosine receptor with C-11 MPDX can be a suitable in vivo imaging technique to evaluate the function of adenosine and adenosine receptor in relation to cerebral ischemia. Less