Investigation for the machamism of hipocampal damage and itsu protection using in vivo and in vitro positron emission tomography study of human brain.

利用人脑体内外正电子发射断层扫描研究研究海马损伤的机制及其保护。

• 批准号: 14571301
• 负责人: NARIAI Tadashi
• 金额: $ 2.24万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571301/
• 关键词: adenosine receptor; hippocampus; positron emission tomography; temporal lobe epilepsy; F-18 FDG; cerebral glucose metabolism; opitical imaging; C11 MPDX; 記憶障害

1) All the patients with intractable temporal lobe epilepsy (TLE) who were operated on in Department of Neurosurgery, Tokyo Medical and Dental University underxvent measurement of cerebral metabolic rate of glucose and the binding potential of central benzodiazepine receptors with positron emission tomography (PET). A new project to image the cerebral adenosine Al receptor using newly developed tracer C-il MPDX and PET in patients with TLE.2) In this new project, we revealed that the adenosine receptor decreased in the fugiform gyrus of focus side and increased in the frontal cortex of focus side. This new finding is now prepared for a paper and a presentation in the international meeting.3) Investitaion to measure the cerebral metabolism of human cortical slice which were obtained in 10 patients with TLE was completed. In this analysis, ~ve revealed that the metabolism in the kalium obtained media corresponded with the metabolism in the preoperative condition measured with PET but that in the control media did not. It suggested that the cerebral metaboliosm in so to speak resting condition is the state that neuron, is working with full neuronal input. This finding is now repared for a paper and a presentation in the international meeting.4) We performed the comparative experiment between the metabolism and neuronal electrical activity in human slice. The latter was performed using optical imaging technique. This experiment revealed that the metabolism is induce by potassium stimulation even in the condition where neuronal conduction is not established. This finding suggest the different behavior of neuron in the slice condition.

1)对在东京医科齿科大学神经外科手术治疗的所有难治性颞叶癫痫（TLE）患者进行了正电子发射断层扫描（PET）脑葡萄糖代谢率和中枢苯二氮卓受体结合电位的测定。一种新的示踪剂C-il MPDX和PET显像TLE患者脑腺苷A1受体的新方案。2）在这个新方案中，我们发现腺苷受体在病灶侧的逃逸回减少，在病灶侧的额叶皮质增加。3）对10例颞叶癫痫患者的脑组织切片进行了脑代谢测定。在这项分析中，~ve显示，钾获得的培养基中的代谢与PET测量的术前条件下的代谢一致，但对照培养基中的代谢不一致。提示静息状态下的脑代谢是神经元在充分的神经元输入下工作的状态。这一发现现已在国际会议上发表论文和报告。4）我们在人体切片上进行了代谢和神经元电活动的比较实验。后者采用光学成像技术进行。本实验表明，即使在神经元传导未建立的条件下，钾刺激也能诱导代谢。这一发现表明了切片条件下神经元的不同行为。

项目成果

Sasaki T, Ishiwata K, Murata T, Senda M.: "Demonstration of competition between endogenous dopamine and [11c]raclopride binding in in vitro brain slices using a dynamic autoradiography technique."Synapse. 44. 42-50 (2002)

Sasaki T、Ishiwata K、Murata T、Senda M.：“使用动态放射自显影技术在体外脑切片中演示内源性多巴胺和[11c]雷氯必利结合之间的竞争。”突触。

Ishiwata K, Nariai T, et al.: "Preclinical studies on [11c]mpdx for mapping adenosine al receptors by positron emission tomography."Ann Nucl Med. 16. 377-382 (2002)

Ishiwata K、Nariai T 等人：“通过正电子发射断层扫描对 [11c]mpdx 绘制腺苷 a1 受体的临床前研究。”Ann Nucl Med。

Shimada Y, Kiyosawa M, Nanai T, Oda K, Toyama H, Ono K, Senda M, Ishiwata K.: "Quantitative in vivo measurement of central benzodiazepine receptors in the brain of cats by use of positron-emission tomography and [11c]flumazenil."Am J Vet Res. 64. 999-1002

Shimada Y、Kiyosawa M、Nanai T、Oda K、Toyama H、Ono K、Senda M、Ishiwata K.：“使用正电子发射断层扫描对猫大脑中的中枢苯二氮卓受体进行体内定量测量和[11c]

Nariai T, Shimada Y, Ishiwata K, Nagaoka T, Shimada J, Kuroiwa T, Ono KI, Ohno K, Hirakawa K, Senda M: "PET imaging of adenosine a(1) receptors with (11)C-MPDX as an indicator of severe cerebral ischemic insult."J Nucl Med. 44. 1839-1844 (2003)

Nariai T、Shimada Y、Ishiwata K、Nagaoka T、Shimada J、Kuroiwa T、Ono KI、Ohno K、Hirakawa K、Senda M：“以 (11)C-MPDX 为指示剂的腺苷 a(1) 受体 PET 成像

Maehara T, Nariai T, Arai N, Kawai K, Shimizu H, Ishii K, Ishiwata K, Ohno K: "Usefulness of [11C]Methionine PET in the Diagnosis of Dysembryoplastic Neuroepithelial Tumor with Temporal Lobe Epilepsy."Epilepsia. 45. 41-45 (2004)

Maehara T、Nariai T、Arai N、Kawai K、Shimizu H、Ishii K、Ishiwata K、Ohno K：“[11C]蛋氨酸 PET 在诊断伴有颞叶癫痫的胚胎发育不良神经上皮肿瘤中的用途。”癫痫。