Expression of death receptor and death ligand in human astrocytic brain tumors

死亡受体和死亡配体在人星形细胞脑肿瘤中的表达

• 批准号: 10671288
• 负责人: TACHIBANA Osamu
• 金额: $ 1.98万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671288/
• 关键词: necrogenesis; apoptosis; glioblastoma; death receptor; caspase-3; 壊死巣; death receptor 5; Caspase-3; PARP

In the present study, I assesed the expression of death receptor, including Fas/APO-1 (CD95), DR4, and DR5, death ligand (Fas ligand, TRAIL), and decoy receptor, and its relation to necrosis phenotype in glioblastomas. I previously reported that Fas expression is predominantly induced in perinecrotic glioma cells, suggesting that Fas induction is associated with apoptosis and necrosis formation, a histological hallmark of glioblastomas. In this study, DR5 expression is induced in large necrosis, like Fas expression. Fas L and TRAIL were expressed in glioblastoma cells. Caspase-3 overexpression and activated appears to correlate with small necrosis area. These results suggested that necrosis phenotype in glioblastoma were different cell death pathway, and small necrosis was dependent to caspase-3 activation and large necrosis dependent to death receptors.

在本研究中，我评估了死亡受体，包括Fas/APO-1 (CD95)、DR4和DR5、死亡配体(Fas配体，TRAIL)和诱饵受体的表达，及其与胶质母细胞瘤坏死表型的关系。我之前报道过 Fas 表达主要在坏死周围胶质瘤细胞中被诱导，这表明 Fas 诱导与细胞凋亡和坏死形成有关，这是胶质母细胞瘤的组织学标志。在这项研究中，DR5 表达在大坏死中被诱导，就像 Fas 表达一样。 Fas L 和 TRAIL 在胶质母细胞瘤细胞中表达。 Caspase-3 过度表达和激活似乎与小坏死区域相关。这些结果表明胶质母细胞瘤的坏死表型是不同的细胞死亡途径，小坏死依赖于caspase-3激活，大坏死依赖于死亡受体。

项目成果

Iwato M, Tachibana O, Tohma Y, Nitta H, Hayashi Y, Yamashita J: "Molecular analysis for p53 and mdm2 in intracranial germ cell tumors."Acta Neuropathol (Berl). 99(1). 21-5 (2000)

Iwato M、Tachibana O、Tohma Y、Nitta H、Hayashi Y、Yamashita J：“颅内生殖细胞肿瘤中 p53 和 mdm2 的分子分析。”Acta Neuropathol (Berl)。