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Gonadotropin-releasing Hormone Receptor and Gonadotropin-releasing Hormone Expression in Human Meningioma Cells

人脑膜瘤细胞中促性腺激素释放激素受体和促性腺激素释放激素表达

基本信息

批准号：
17591507
负责人：
TACHIBANA Osamu
金额：
$ 2.11万
依托单位：
Kanazawa Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Gonadotropin-releasing hormone (GnRH) and its receptor (GnRH-R) are present in a number of hormone-dependent tumors, including cancers of breast, endometrium, and prostate. An autocrine regulatory system based on GnRH makes a part of cell proliferation. In the present study, the expression of GnRH-R and GnRH were investigated using immunohistochemical analysis in intracranial meningiomas from 82 patients. Reverse transcription-PCR was performed to detect the expression of GnRH-R and GnRH mRNA in 21 cases. Statistical differences between immunoreactivity for GnRH-R and estrogen receptor labeling index (LI), progesteron receptor LI, Ki-67 LI or clinicopathological parameters were evaluated. To elucidate the functional role of GnRH and GnRH-R in meningioma cell proliferation assays in 6 cultured meningioma cells treated with GnRH agonist and antagonist were performed. The expression of GnRH-R and GnRH mRNA was detected in 100% and 86% of all cases. Immunoreactivity for GnRH-R and GnRH was found in 95% and 60% of 21 cases. There was a positive correlation between GnRH-R immunoreactivity and PgR LI. GnRH-positive cases were found more in men than women. No significant differences were detected between GnRH-R or GnRH and other parameters. A significant proliferation was detected in treatment of all 6 cultured cells with GnRH agonist in comparison with control cultures. GnRH agonist and antagonist had no effect in all 3 cultured cells. In treatment of an only case that was GnRH-positive with antagonist alone, a significant inhibition of growth was detected. It suggests that there may be an autocrine system in the meningiomas and GnRH-R and GnRH acts as a regulator of cell proliferation.

促性腺激素释放激素（GnRH）及其受体（GnRH-R）存在于许多依赖激素的肿瘤中，包括乳腺癌、子宫内膜癌和前列腺癌。基于GnRH的自分泌调节系统是细胞增殖的一部分。本研究应用免疫组化方法检测82例颅内脑膜瘤中GnRH-R和GnRH的表达。采用逆转录-聚合酶链反应（RT-PCR）检测21例患者的GnRH-R和GnRHmRNA的表达。比较GnRH-R免疫反应性与雌激素受体标记指数（LI）、雌激素受体LI、Ki-67 LI或临床病理参数的差异。为探讨GnRH和GnRH-R在脑膜瘤细胞增殖中的作用，用GnRH激动剂和拮抗剂处理6个培养的脑膜瘤细胞，进行细胞增殖实验。GnRH-R和GnRHmRNA的表达率分别为100%和86%。21例中GnRH-R和GnRH免疫反应阳性率分别为95%和60%。GnRH-R免疫反应性与PgR LI呈正相关。GnRH阳性病例男性多于女性。GnRH-R或GnRH与其他参数之间未检测到显著差异。与对照培养物相比，在用GnRH激动剂处理所有6个培养的细胞中检测到显著的增殖。GnRH激动剂和拮抗剂对3种细胞均无作用。在仅用拮抗剂治疗GnRH阳性的唯一病例中，检测到显著的生长抑制。提示脑膜瘤中可能存在自分泌系统，GnRH-R和GnRH对细胞增殖起调节作用。