Gonadotropin-releasing Hormone Receptor and Gonadotropin-releasing Hormone Expression in Human Meningioma Cells

促性腺激素释放激素（GnRH）及其受体（GnRH- r）存在于许多激素依赖性肿瘤中，包括乳腺癌、子宫内膜癌和前列腺癌。基于GnRH的自分泌调节系统是细胞增殖的一部分。本研究采用免疫组化方法对82例颅内脑膜瘤患者的GnRH- r和GnRH的表达进行了研究。采用逆转录pcr检测21例患者GnRH- r及GnRH mRNA的表达。评价GnRH-R免疫反应性与雌激素受体标记指数（LI）、孕激素受体LI、Ki-67 LI及临床病理参数的差异。为了阐明GnRH和GnRH- r在脑膜瘤细胞增殖中的功能作用，对6个培养的脑膜瘤细胞进行了GnRH激动剂和拮抗剂处理。GnRH- r和GnRH mRNA的表达在所有病例中分别为100%和86%。21例患者中GnRH- r和GnRH的免疫反应性分别为95%和60%。GnRH-R免疫反应性与PgR LI呈正相关。gnrh阳性病例男性多于女性。GnRH- r及GnRH与其他参数无显著差异。与对照培养物相比，在GnRH激动剂处理的所有6个培养细胞中均检测到显著的增殖。GnRH激动剂和拮抗剂对3种培养细胞均无影响。在仅用拮抗剂治疗gnrh阳性的病例中，检测到显著的生长抑制作用。提示脑膜瘤中可能存在自分泌系统，GnRH- r和GnRH作为细胞增殖的调节因子。

Gonadotropin-releasing hormone (GnRH) and its receptor (GnRH-R) are present in a number of hormone-dependent tumors, including cancers of breast, endometrium, and prostate. An autocrine regulatory system based on GnRH makes a part of cell proliferation. In the present study, the expression of GnRH-R and GnRH were investigated using immunohistochemical analysis in intracranial meningiomas from 82 patients. Reverse transcription-PCR was performed to detect the expression of GnRH-R and GnRH mRNA in 21 cases. Statistical differences between immunoreactivity for GnRH-R and estrogen receptor labeling index (LI), progesteron receptor LI, Ki-67 LI or clinicopathological parameters were evaluated. To elucidate the functional role of GnRH and GnRH-R in meningioma cell proliferation assays in 6 cultured meningioma cells treated with GnRH agonist and antagonist were performed. The expression of GnRH-R and GnRH mRNA was detected in 100% and 86% of all cases. Immunoreactivity for GnRH-R and GnRH was found in 95% and 60% of 21 cases. There was a positive correlation between GnRH-R immunoreactivity and PgR LI. GnRH-positive cases were found more in men than women. No significant differences were detected between GnRH-R or GnRH and other parameters. A significant proliferation was detected in treatment of all 6 cultured cells with GnRH agonist in comparison with control cultures. GnRH agonist and antagonist had no effect in all 3 cultured cells. In treatment of an only case that was GnRH-positive with antagonist alone, a significant inhibition of growth was detected. It suggests that there may be an autocrine system in the meningiomas and GnRH-R and GnRH acts as a regulator of cell proliferation.