No Invasive Methods For Bone quality Using Superconduction Quantum Interference Device

我们尝试用超导量子干涉仪测量脉冲电磁刺激（10、20、30 Gaus, 2Hz）后兔腿的反应性电磁场。但由于软组织（神经、肌肉等）产生的脉冲电磁场、反应磁场中存在大量噪声，因此无法检测到骨组织产生的反应磁场。接下来我们尝试评估骨组织在愈伤组织形成过程中的阻抗。我们使用30只家兔胫骨长骨痂。30只日本大白兔分为3组：ES组（早期刺激，术后交替刺激）、LS组（晚期刺激，术后3周电刺激）和C组（对照组）。等待期为1周，延长率为0.5mm，每日2次。在延长完成后2周处死的动物进行放射学、组织学和电生理研究。影像学上ES组和LS组骨皮质化和髓质化开始早于c组，ES组和LS组骨矿物质密度较c组显著降低，ES组切除胫骨阻抗值较c组显著升高，且阻抗值随延长的骨痂成熟而升高。当电流路径由骨和软组织组成一个简单的并联电路时，我们认为整个电流中约有64%通过骨组织。交流电刺激延长愈伤组织有利于矿化愈伤组织的重塑，延长愈伤组织的阻抗反映了愈伤组织的成熟过程。

We tried to measure a reactive electricmagnetic field of a leg in rabbit after pulsing electromagnetic stimulation (10, 20, 30 Gaus, 2Hz) using Superconduction Quantum Interference Device. However, we had a lot of noise composed of the pulsing electromagnetic field, reactive magnetic field from soft tissues (nerve, muscle etc.), Therefore, we can not detect the reactive magnetic field from bone tissue. Next we try to evaluate the impedance of bone tissue in the process of callus formation. We used lengthend callus of tibiae in 30 rabbits. Thirty Japanese white rabbits were divided into 3 groups : Group ES (early stimulation ; Alternating stimulation from just after operation), Group LS (late stimulation ; electrical stimulation from 3 weeks after operation) Group C (control). The waiting period was 1 week and the lengthening rate was 0.5mm twice daily. Radiological, histological, and electrophysiological studies were done on animals sacrificed at 2 weeks after the completion of lengthening. Radiologically in groups ES and LS, corticalization and medullarization began earlier than in group C.The values of bone mineral density of the lengthened callus in groups ES and LS were significantly decreased as compared with those in group C.The impedance values for the resected tibia in group ES were significantly increased, as compared to those in group C.The impedance values of resected tibia increased with the maturation of the lengthened callus. When electrical current route was composed of bone and soft tissue as a simple parallel circuit, we considered that about 64% of the whole electrical current passed through bone tissue. Alternating current electrical stimulation to the lengthened callus was useful for remodeling of the mineralized callus, and the impedance of lengthened callus reflected the maturation itself.