Analysis of response mechanism of osteoblast by physical stimulation -mechanical stress and electrical stimulation

我们对成骨细胞系MC3T3-E1进行交流电刺激，并以MAPK(丝裂原活化蛋白激酶)的磷酸化为指标。交流电刺激20min后，ERK和p38(MAPK)的磷酸化水平明显高于未刺激组。部分对成骨细胞电刺激的反应机制进行了阐明，建立了有用的机械应力刺激条件和方法。

We conducted an alternating current electrical stimulation to theosteoblastic cell line MC3T3-E1, and examined the phosphorylation of MAPK(mitogen-activated protein kinase) as an indicator . By the AC electrical stimulation for 20 min, phosphorylation of ERK and p38 (MAPK) was increased significantly than thenon-stimulated group. Part of the response mechanism for electrical stimulation of osteoblasts been elucidated, the establishment of stimulus conditions and methods of useful mechanical stress can be expected.