Investigation of nitric oxide production control by pain stimulation in the rat spinal cord.

通过大鼠脊髓疼痛刺激控制一氧化氮产生的研究。

• 批准号: 10671397
• 负责人: SAITOH Shigeyuki
• 金额: $ 1.86万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671397/
• 关键词: nitric oxide; spinal cord; pain; Rat; electrode; 電極法; 疼痛刺激

Investigation of nitric oxide production control by pain stimulation in the rat spinal cord.We investigate the nitric oxide production control by pain stimulation in the rat spinal cord.There is a great deal of evidence that nitric oxide (NO) plays a role in synaptic transmission in both the central and peripheral nervous systems. NO is a short-lived radical involved in various biological processes. There are several reports that peripheral pain stimulation transmission to the central was mediated by NO in the spinal level. The evidence was proved by using nitric oxide antagonists such as L-NAME indirectly.We have used an electrochemical microsensor to measure nitric oxide (NO) directly by pain stimulation in rat spinal canal.The nitric oxide sensitive electrode was used to measure the change of nitric oxide in the lumbar spinal canal in pentobarbital anesthetized rat.Result. NO signals were augmented by pain stimulation, and attenuated by an inhibitor of nitric oxide synthase L-NAME.The results provide the evidence that nitric oxide is produced by pain stimulation and gave a method for measure the nitric oxide in the spinal canal.

疼痛刺激对大鼠脊髓中一氧化氮产生的控制研究我们研究了疼痛刺激对大鼠脊髓中一氧化氮产生的控制，大量证据表明一氧化氮（NO）在中枢和外周神经系统的突触传递中起作用。NO是一种参与各种生物过程的短寿命自由基。有几份报告表明，外周疼痛刺激传递到中枢是由脊髓水平的NO介导的。本实验采用电化学微传感器直接测定大鼠椎管内一氧化氮（NO）的含量，并采用一氧化氮敏感电极测定戊巴比妥钠麻醉大鼠腰椎椎管内NO含量的变化。疼痛刺激可使NO信号增强，一氧化氮合酶抑制剂L-NAME可使NO信号减弱，为疼痛刺激产生一氧化氮提供了证据，并为椎管内一氧化氮的测定提供了方法。

项目成果

Fukuda T.,Saito S.: "Hal****M : n mu A-1 ce** Awe3 th** Concentration a** Neuronal Nitric Oxidesynd*ha*e activity of the Dors*l Horn and the Lou*s Cesule**"Are*th. Anclg. 89. 1035-1039 (1999)

Fukuda T.，Saito S.：“Hal****M：n mu A-1 ce** Awe3 th** 浓度 a** 神经元一氧化氮合成*ha*e 背*l 角和楼*的活动*

Fukuda T, Saito S, Sato S, Harukuni I, Toyooka H.: "Halothane minimum alveolar anesthetic concentration and neuronal nitric oxide synthase activity of the dorsal horn and the kocus ceruleus in rats"Anesth Analg. 89. 1035-9 (1999)

Fukuda T、Saito S、Sato S、Harukuni I、Toyooka H.：“氟烷最低肺泡麻醉浓度和大鼠背角和蓝斑的神经元一氧化氮合酶活性”Anesth Analg。