Acute rejection of urinary bladder tranplantation in rats

大鼠膀胱移植的急性排斥反应

• 批准号: 10671480
• 负责人: OHOKA Hiroji
• 金额: $ 1.22万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671480/
• 关键词: bladder transplantation; acute rejection; apoptosis; rat

The male Lewis rats weighting 250 to 350 g were used for donors and the male BN rats weighting 250 to 350 g recipients were used to confirm a form of acute rejection of urinary bladder transplantation. After transplantation no immunosuppressive drugs were administrated. Transplanted bladder and common bladder were removed on the 4ィイD1thィエD1, 6ィイD1thィエD1, 8ィイD1thィエD1, 10ィイD1thィエD1, 12ィイD1thィエD1, and 14ィイD1thィエD1 day and the histological change was observed. The existence of apoptosis was confirmed with tunnel method for the specimens on the 8ィイD1thィエD1 day.On the 6ィイD1thィエD1 day, all cases of the blood supply to the graft were kept, but on the 8ィイD1thィエD1 day they were obstructed. On the 4ィイD1thィエD1 day, infiltration of the inflammatory cells to the epithelium and muscle were very slight and a construction was kept normally. On the 6ィイD1thィエD1 day, infiltration of the inflammatory cells to the submucosa was observed. On the 8ィイD1thィエD1 day, infiltration of the inflammatory cells and bleeding in the epithelium were observed and infiltration of the lymphocyte was remarkable. The existence of apoptosis in the epithelium was confirmed with tunnel method. On the 10ィイD1thィエD1 day, the epithelium was dropping away and construction of the muscle was destroyed. On the 12ィイD1thィエD1 day, the epithelium was disappeared completely, the submucosa and the muscle were necrotic.

将体重250 - 350 g的雄性刘易斯大鼠用作供体，将体重250 - 350 g的雄性BN大鼠用作受体以确认膀胱移植的急性排斥形式。移植后未给予免疫抑制剂。分别于术后第4天、第6天、第8天、第10天、第12天、第14天取出移植膀胱和普通膀胱，观察组织学变化。第8周D1 ~ D1 d的标本用隧道法证实细胞凋亡的存在，第6周D1 ~ D1 d的移植物血供全部保持，第8周D1 ~ D1 d的移植物血供全部阻断。在第4天、第1天、第1天，炎性细胞向上皮和肌肉的浸润非常轻微，结构保持正常。在第6天第1天，观察到炎性细胞浸润到粘膜下层。术后第8天，上皮内可见炎性细胞浸润和出血，淋巴细胞浸润明显。隧道法证实上皮细胞凋亡的存在。第10天，上皮脱落，肌肉结构破坏。第12天，粘膜上皮完全消失，粘膜下层和肌肉坏死。