Bladder Transplantation : Histological Findings

膀胱移植：组织学结果

• 批准号: 06807126
• 负责人: OHOKA Hiroji
• 金额: $ 1.28万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06807126/
• 关键词: bladder transplantation; rat; microsurgery; ラット動物実験モデル

Patients who require cystectomy are usually treated by ileal conduit or intestinal neobladder for urinary control. In some of them, however, the bowel segment cannot be used because of previous abdominal surgery or radiation treatment. Bladder transplantation from cadavers may be beneficial to these patients, if possible. In order to obtain basic knowledge about bladder transplantation (TPL), we developed an animal model of whole bladder transplantation in rats. Male Lewis rats weighing 270-320g were used as both donors and recipients. Of the 23 recipients, 12 (52.2%) survived 7 days or longer postoperatively. At 1 weeks after TPL,the bladder showed loss of transitional epithelium and remarkable cellular infiltration. In the bladder at 5 weeks after TPL the transitional epithelium regenerated markedly and submucosal cellular infiltration was much improved. Regeneration of some smooth muscle cells was also noted. At 6 months after TPL,the nerve fibers were recognized in the bladder and the volume of the tranaplanted bladder was well preserved (1.0-1.3ml).

需要膀胱术的患者通常采用回肠导管或肠代膀胱术进行排尿控制。然而，在其中一些患者中，由于先前的腹部手术或放射治疗，肠段不能使用。如果可能的话，尸体膀胱移植可能对这些患者有益。为了获得膀胱移植的基本知识，我们建立了大鼠全膀胱移植的动物模型。体重270- 320 g的雄性刘易斯大鼠用作供体和受体。23例受者中，12例（52.2%）术后存活7天或以上。TPL后1周，膀胱移行上皮消失，细胞浸润明显。TPL后5周膀胱移行上皮再生明显，粘膜下细胞浸润明显改善。还观察到一些平滑肌细胞再生。TPL术后6个月，膀胱内神经纤维清晰可见，移植膀胱体积保持良好（1.0-1.3ml）。