权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study on the significance and control mechanism of apoptosis in follicle atresia system

卵泡闭锁系统细胞凋亡的意义及调控机制研究

基本信息

批准号：
10671548
负责人：
IRAHARA Minoru
金额：
$ 2.11万
依托单位：
University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

In the present project, to study on the significance and control mechanism of apoptosis in follicle atresia system, I investigated (1) the existence of interleukin-1 beta converting enzyme (ICE), which is a key enzyme of apoptosis, (2) the changes of ICE in ovulation system in vivo and in vitro, (3) the control factor of ICE in vitro, using measurement of ICE mRNA in rats ovaries and its culture system.1) ICE mRNA was detected in rat ovaries. Its amount in ovary was 10% of that in spleen.2) In PMSG-hCG treatment, ICE mRNA in ovary was not changed in PMSG injection, but its appearance increased immedately 6 hours after hCG injection, reached to the peak 24 hours after (152% increase of pre-treatment), and retured to pre-treatment level 48 hours after.3) GnRH, androgen and TNF-alph atimulated ICE mRNA expression in ovary (140%, 160% and 200% of control, respectiveky). However, dexamethasone inhibited ICE mRNA expression (60% of control).In conclusion, I demonstrated that (1) ICE, which is a key enzyme of appearane of apoptosis in Fas-Fas ligand system, exists in ovary, (2) ICE is deeply concerned with ovulation system, (3) GnRH, androgen and TNF-alph, which are reported as stimulating factors of apoptosis in follicle atresia, stimulate ICE expression in ovary. These data indicate that apoptosis process via ICE system has important roles in follicle atresia system.

在本项目中，为了研究卵泡闭锁系统中细胞凋亡的意义和控制机制，我通过测量大鼠中的ICE mRNA，研究了（1）细胞凋亡关键酶白介素-1β转换酶（ICE）的存在，（2）体内和体外排卵系统中ICE的变化，（3）体外ICE的控制因子卵巢及其培养体系。1)在大鼠卵巢中检测到ICE mRNA。其在卵巢中的量为脾脏中的10%。2)在PMSG-hCG治疗中，PMSG注射后卵巢中的ICE mRNA没有变化，但在注射hCG后6小时其出现立即增加，24小时后达到峰值(治疗前增加152%)，并在48小时后恢复到治疗前水平。3)GnRH、雄激素和TNF-α刺激ICE信使RNA 卵巢中的表达（分别为对照的 140%、160% 和 200%）。然而，地塞米松抑制了ICE mRNA表达（对照的60%）。总之，我证明了（1）卵巢中存在ICE，它是Fas-Fas配体系统中细胞凋亡出现的关键酶，（2）ICE与排卵系统密切相关，（3）GnRH，雄激素和TNF-α，据报道是卵泡细胞凋亡的刺激因子闭锁，刺激卵巢中的 ICE 表达。这些数据表明，通过ICE系统的细胞凋亡过程在卵泡闭锁系统中具有重要作用。