Development of safe and convenient ovulation induction

开发安全便捷的促排卵剂

• 批准号: 17591744
• 负责人: IRAHARA Minoru
• 金额: $ 2.24万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591744/
• 关键词: ovulation induction; low-dose FSH therapy; FSH-pulsatile GnRH therapy; self injection; metformin; polycystic ovary syndrome; safety; convenience

Ovulation induction using gonadotropin for infertile women requires daily attendance to hospital for injection, and is highly associated with multiple pregnancies. We developed and evaluated new method to induce ovulation: FSH-pulsatile GnRH(F-G), chronic low-dose FSH self-injection(LD-F) and clomiphene-metformine(Cl-Met). Study designs were prospective randomized and cross over in all investigations.1. F-GWe compared efficacy and safety between F-G and LD-F in 10 PCOS patients. Schedule of LD-F: Daily injection of FSH was started with a dose of 75 IU and increased after 14 days treatment in case no follicle developed.Schedule of F-G: Daily injection of FSH was started with a dose of 150 IU and switched to pulsatile GnRH administration when follicle reached 11mm. Both treatments were equally effective. Total day of attendance to hospital per cycle was significantly shorter in F-G(8.8± 1.3 days, mean±SE) than in LD-F(17.7± 6.6) (P<0.01).2. LD-F by self-injectionLD-F was performed by self-injection(SI) or by attendance to hospital(AH) to 3 anovulatory patients. All cycles of both treatment achieved single ovulation, and no side effect was observed. Total days of attendance to follicular maturation were 6.0 ±1.0 clays in SI and 17.7± 8.0 days in AH (p=0.067).3. Cl-MetWe compared efficacy and safety between Cl and Cl-Met in 5 clomiphene (100mg)-resistant women with PCOS. Dose of clomiphene was 150 mg/day and dose of metformin was 750 mg/day(day5 to follicular maturation). One out of 4 in Cl, and 4 out of 5 in Cl-Met showed ovulation. All cycles achieved single ovulations. Pregnancies were achieved in 2 of Cl-Met(singletons).In conclusion, all of new methods were safe and comprehensive methods for infertile patients.

对于不孕女性使用促性腺激素诱导排卵需要每天去医院注射，并且与多胎妊娠高度相关。我们开发并评估了诱导排卵的新方法：FSH脉冲促性腺激素释放激素（F-G）、慢性低剂量FSH自我注射（LD-F）和克罗米芬二甲双胍（Cl-Met）。研究设计是前瞻性随机的，并在所有调查中交叉。1． F-GWe 比较了 F-G 和 LD-F 在 10 名 PCOS 患者中的疗效和安全性。 LD-F方案：每日注射FSH，剂量为75 IU，治疗14天后如果没有卵泡发育，则增加剂量。F-G方案：每日注射FSH，剂量为150 IU，当卵泡达到11mm时转为脉冲式GnRH给药。两种治疗方法同样有效。 F-G每个周期住院总天数（8.8±1.3天，平均值±SE）明显短于LD-F（17.7±6.6）（P<0.01）。2．自我注射LD-F LD-F通过自我注射(SI)或住院(AH)对3名无排卵患者进行。两种治疗的所有周期均实现单次排卵，且未观察到副作用。 SI 中卵泡成熟的总天数为 6.0 ±1.0 天，AH 中为 17.7±8.0 天 (p=0.067).3。 Cl-Met 我们比较了 Cl 和 Cl-Met 在 5 名患有 PCOS 的克罗米芬 (100mg) 耐药女性中的疗效和安全性。克罗米芬的剂量为150mg/天，二甲双胍的剂量为750mg/天（卵泡成熟第5天）。 Cl 中四分之一的细胞和 Cl-Met 中五分之四的细胞显示排卵。所有周期均实现单次排卵。 2例Cl-Met（单胎）成功怀孕。综上所述，所有新方法对于不孕症患者都是安全且全面的方法。

项目成果

Myeloid-related protein-8/14 is associated with proinflammatory cytokines in cervial mucus.

骨髓相关蛋白 8/14 与宫颈粘液中的促炎细胞因子相关。

• 发表时间: 2006
• 期刊: J Reprod Immunol. 71・1
• 作者: Okegawa T;Pong RC;Li Y;Hsieh JT.;Iwasa T;Takeshi Iwasa;Junko Komatsu;Takeshi Iwasa;Komatsu J;Iwasa T;Kinoshita D;Nakagawa K;Kunimi K;Yasui T;Nakagawa K;Dan Kinoshita;Koji Nakagawa;Kotarou Kunimi
• 通讯作者: Kotarou Kunimi

Reduction of perifollicular arterial blood flow resisteance after hCG administration is a good indicator of the recovery of mature oocytes in ART treatment.

施用 hCG 后卵泡周围动脉血流阻力的降低是 ART 治疗中成熟卵母细胞恢复的良好指标。

• 发表时间: 2006
• 期刊: J Assist Reprod Genet. 23・11-12
• 作者: Okegawa T;Pong RC;Li Y;Hsieh JT.;Iwasa T;Takeshi Iwasa;Junko Komatsu;Takeshi Iwasa;Komatsu J;Iwasa T;Kinoshita D;Nakagawa K;Kunimi K;Yasui T;Nakagawa K;Dan Kinoshita;Koji Nakagawa;Kotarou Kunimi;Toshiyuki Yasui;Koji Nakagawa
• 通讯作者: Koji Nakagawa

The signaling pathways linking to lysophosphatidic acid-promoted meiotic maturation in mice

• DOI: 10.1016/j.lfs.2006.01.028
• 发表时间: 2006-06-27
• 期刊: LIFE SCIENCES
• 影响因子: 6.1
• 作者: Komatsu, Junko;Yamano, Shuji;Irahara, Minoru
• 通讯作者: Irahara, Minoru

Comparison and Problems of Measured Values of LH, FSH, and PRL among Measurement Systems

LH、FSH、PRL测量系统测量值的比较及问题

• 发表时间: 2006
• 期刊: Endocr J 53・1
• 作者: Okegawa T;Pong RC;Li Y;Hsieh JT.;Iwasa T;Takeshi Iwasa;Junko Komatsu;Takeshi Iwasa
• 通讯作者: Takeshi Iwasa

Intraovarian arterial blood flow resistance in oligomenorrheal infertile women.

月经稀发不孕妇女的卵巢内动脉血流阻力。

• 发表时间: 2006
• 期刊: J Assist Reprod Genet. 23(3)
• 作者: Okegawa T;Pong RC;Li Y;Hsieh JT.;Iwasa T;Takeshi Iwasa;Junko Komatsu;Takeshi Iwasa;Komatsu J;Iwasa T;Kinoshita D;Nakagawa K
• 通讯作者: Nakagawa K