The study on the etiological meaning of oligosaccharide residures in immunological infertility and development of treatment for that infertility.

本文用体外成熟人透明精子穿透试验方法，对不孕妇女血清中精子固定抗体的病因学意义进行了论证。本课题研究了抗精子固定抗体抗原的病因学意义，特别是寡糖残基在精卵相互作用中的作用。（方法）采用体外成熟人透明带精子固定试验（SIT）和穿透试验（SPT）。（结果）（1）从WHO库血清和几种不孕症患者血清中筛选出具有SIT精子固定活性的高效价血清。所有收集的血清均阻断精子穿透和附着于人透明膜。结果表明，精子固定抗体具有阻断精子穿透精子和受精的作用。(2)我们用几种凝集素进行了SIT，证明了刀豆球蛋白A（C 关于我们 在A）上，麦胚凝集素（WGA）和蓖麻凝集素（RCA）具有SIT的阻断活性。(3)我们检测了这三种凝集素在人精子和透明精子上是否存在寡糖残基。用荧光技术检测了精子和卵母细胞表面的寡糖。(4)我们研究了添加这些寡糖残基对经凝集素处理的无精子穿透活性的卵母细胞的精子穿透活性的恢复。从该结果可知，这3种凝集素中只有甘露糖一种凝集素具有恢复精子穿透的作用。证明了甘露糖在精卵相互作用中的重要作用。(5)另一方面，研究了几种寡糖对精子穿透能力的影响。我们还发现只有甘露糖具有精子穿透的阻断活性。这种活性是可逆的，与精子顶体反应无关。（结论）精子和卵子上的寡糖残基在精卵相互作用中起重要作用，对免疫性不育的病因学有一定的意义。并发现低聚糖对这种免疫性不孕症有治疗作用。少

We have demonstrated that the etiological meaning of sperm immobilizing antibodies in sera of infertile women using the method of sperm penetration test using human zona pellucida matured in vitro. In this project, we studied on the etiological meaning of the antigens against those sperm immobilizing antibodies, especially the role of oligosaccharide residues in sperm-egg interaction.(Methods) Sperm immobilizing test (SIT) and sperm penetration test using human zona pellucida matured in vitro (SPT) were used.(Results) (1) We picked up the high titer sera with sperm immobilizing activities by SIT from WHO bank sera and the sera of some kinds of infertile women. All of the picked up sera blocked sperm penetration and attachment to human zona pellucida. From this result, we demonstrated that sperm immobilizing antibodies have the blocking activities of sperm penetration to zona and fertilization. (2) We performed the SIT using some kinds of lectins, and demonstrated that Concanavalin A (C … More on A), Wheat germ agglutinin (WGA) and Ricinus communis agglutinin (RCA) have the blocing activities of SIT. (3) We examined the existence of the oligosaccharide residures against these 3 lectins on human sperm and zona pellucida. We found the existence of these oligosaccharides on the surface of sperm and zona pellusida by fluoresent technique. (4) We studied the recovery of sperm penetration activities by addition of these oligosaccharide residues on the oocytes that were treated by lectins and had no activity of sperm penetration. From this result, only one lectin of these 3 lectins, mannose, had the effect of recovery of sperm penetration. We demonstrated that mannose have the important role on sperm-egg interaction. (5) On the other hand, we examined the change of activity of sperm penetration when sperm were pre-incubated with some kinds of oligosaccharides. We also found that only mannose have the blocking activity of sperm penetration. This activites were reversible and were not concerned with sperm acrosome reaction.(Conclusion) These date indicated that the oligosaccharide residues on sperm and egg have the important role on sperm-egg interaction, and have the etiological meaning in immunological infertility. And we found that oligosaochride had posibility of treatment for this immunological infertility using oligosaccharides. Less