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The study on the etiological meaning of oligosaccharide residures in immunological infertility and development of treatment for that infertility.

低聚糖残留在免疫性不孕症中的病因学意义及其治疗进展的研究。

基本信息

批准号：
62570759
负责人：
IRAHARA Minoru
金额：
$ 1.34万
依托单位：
University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

We have demonstrated that the etiological meaning of sperm immobilizing antibodies in sera of infertile women using the method of sperm penetration test using human zona pellucida matured in vitro. In this project, we studied on the etiological meaning of the antigens against those sperm immobilizing antibodies, especially the role of oligosaccharide residues in sperm-egg interaction.(Methods) Sperm immobilizing test (SIT) and sperm penetration test using human zona pellucida matured in vitro (SPT) were used.(Results) (1) We picked up the high titer sera with sperm immobilizing activities by SIT from WHO bank sera and the sera of some kinds of infertile women. All of the picked up sera blocked sperm penetration and attachment to human zona pellucida. From this result, we demonstrated that sperm immobilizing antibodies have the blocking activities of sperm penetration to zona and fertilization. (2) We performed the SIT using some kinds of lectins, and demonstrated that Concanavalin A (C … More on A), Wheat germ agglutinin (WGA) and Ricinus communis agglutinin (RCA) have the blocing activities of SIT. (3) We examined the existence of the oligosaccharide residures against these 3 lectins on human sperm and zona pellucida. We found the existence of these oligosaccharides on the surface of sperm and zona pellusida by fluoresent technique. (4) We studied the recovery of sperm penetration activities by addition of these oligosaccharide residues on the oocytes that were treated by lectins and had no activity of sperm penetration. From this result, only one lectin of these 3 lectins, mannose, had the effect of recovery of sperm penetration. We demonstrated that mannose have the important role on sperm-egg interaction. (5) On the other hand, we examined the change of activity of sperm penetration when sperm were pre-incubated with some kinds of oligosaccharides. We also found that only mannose have the blocking activity of sperm penetration. This activites were reversible and were not concerned with sperm acrosome reaction.(Conclusion) These date indicated that the oligosaccharide residues on sperm and egg have the important role on sperm-egg interaction, and have the etiological meaning in immunological infertility. And we found that oligosaochride had posibility of treatment for this immunological infertility using oligosaccharides. Less

我们利用体外成熟的人透明带进行精子穿透试验的方法，证明了不育妇女血清中精子固定抗体的病因学意义。在本项目中，我们研究了针对这些精子固定化抗体的抗原的病因学意义，特别是寡糖残基在精卵相互作用中的作用。（方法）采用精子固定化试验（SIT）和体外成熟的人透明带精子穿透试验（SPT）。（结果）（1）通过 SIT 来自 WHO 库血清和某些不孕妇女的血清。所有收集到的血清都会阻止精子穿透并附着在人类透明带上。由此结果表明，精子固定化抗体具有阻碍精子穿入透明带和受精的活性。 (2)我们使用几种凝集素进行了SIT，并证明了伴刀豆球蛋白A（C … More on A）、小麦胚芽凝集素（WGA）和蓖麻凝集素（RCA）具有SIT的凝集活性。 (3)我们检测了人精子和透明带上是否存在针对这3种凝集素的寡糖残基。我们通过荧光技术发现精子和透明带表面存在这些寡糖。 (4)我们研究了通过在经凝集素处理且无精子穿透活性的卵母细胞上添加这些寡糖残基来恢复精子穿透活性。从该结果来看，这3种凝集素中只有一种凝集素，即甘露糖，具有恢复精子穿透力的作用。我们证明甘露糖在精子-卵子相互作用中发挥重要作用。 (5)另一方面，我们检测了精子与某些低聚糖预孵育时精子穿透活性的变化。我们还发现，只有甘露糖具有阻断精子穿透的活性。该活性是可逆的，与精子顶体反应无关。(结论)这些研究结果表明，精子和卵子上的寡糖残基在精卵相互作用中发挥着重要作用，在免疫性不孕症中具有病因学意义。我们发现寡糖有可能利用寡糖治疗这种免疫性不孕症。较少的