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Development of small-caliber vascular grafts for free tissue transfer

开发用于自由组织移植的小口径血管移植物

基本信息

批准号：
10671688
负责人：
NOZAKI Motohiro
金额：
$ 1.15万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

This research reported that experimental study of small-caliber vascular grafts shown 1-2mm in diameter could be utilized in the vascular pedicle of the free tissue transfer as a donor for reconstruction after malignant tumor resection. The small-diameter artificial blood vessel was made of the polyurethane (l.4mm inside diameter, 15mm length), and the inner surface was coated with 2-Hydroxyethyl methacrylate-Styrene block-copolymer and Argatroban contained urethane solution. The experimental model in the rabbit was an inferior epigastric flap, which had the vascular pedicle of the femoral and inferior epigastrice artery and vein. And it anastmosed to the renal artery and vein which were located in the retroperitoneal space. The transplantation of the epigastric flap was performed using the artificial vessels as connectors of flap and renal side. The following experiments were done : Acute experiments by non-coated and coated artificial vessls (n=3) and chronic experiments (n=17) by coated grafts. As a result in acute erxperiment, non-coated artificial vessel was thrombosed in all cases within 3 hours. In the chronic experiment, 1 week flap survival existed in 10 flaps (59%) in the 17 cases. The grafts of both artery and venous side were patent in 3 cases. The angiogenesis between the flap and recipient bed of retroperitoneum was observed to occur within on week. Since in this model the graft was just inserted and fixed to the blood vessel, the thrombosis based on the endothelium hyperplasia in artificial blood vessel occurred and occluded. However, it seemed to show the future prospect, which applied small-diameter artificial blood vessels to free tissue transfer in order to decrease the sacrifice of the donor site.

本研究报告了在恶性肿瘤切除术后，将直径为1- 2 mm的小口径血管移植于游离组织移植的血管蒂，作为重建的供体的实验研究。小直径人工血管由聚氨酯制成（内径1.4mm，长度15 mm），内表面涂有甲基丙烯酸2-羟乙酯-苯乙烯嵌段共聚物和含阿加曲班的聚氨酯溶液。实验模型为以股动脉和腹壁下动、静脉为蒂的腹壁下皮瓣。与腹膜后间隙的肾动、静脉吻合。以人工血管为皮瓣与肾侧的连接点，进行上腹部皮瓣移植。进行了以下实验：无涂层和涂层人工血管的急性实验（n=3）和涂层移植物的慢性实验（n=17）。急性实验中，所有病例的无涂层人工血管均在3小时内形成血栓。慢性实验中17例中10例（59%）皮瓣成活1周。3例动静脉侧桥血管均通畅。术后1周内观察到腹膜后皮瓣与受体床间血管生成。由于在该模型中，移植物只是插入并固定在血管上，因此在人工血管中发生基于内皮增生的血栓形成并闭塞。但将小口径的人工血管应用于游离组织移植，以减少供区的牺牲，似乎显示了未来的前景。