Architecture of lymphatic vessels on the head and neck region. -Computer graphic three dimensional reconstruction-

每个器官都有自己的淋巴结构。我们将在这张海报中展示过去三年使用CG 3D重建方法对头颈部淋巴结构的研究结果。[材料与方法]取18天龄小鼠胎仔，用10%中性福尔马林固定。脱水后，将样品包埋在GMA树脂中。制作3 μm连续切片，甲苯胺蓝染色。通过3CCD摄像机将每个切片的二维图像输入计算机，并通过Photoshop（Adobe）提取淋巴管和其他结构。这些图像通过Voxblast（VayTek）重建成三维图像。[结果]舌：舌背各结缔组织乳头均有盲端毛细淋巴管进入。舌下表面毛细淋巴管网络粗糙，无盲端。下颌骨：下颌孔有集合淋巴管，颏孔无集合淋巴管。下颌管内淋巴管与下牙槽动脉伴行，与下牙槽静脉不伴行。食管：有三个淋巴网络。第一个位于固有层，有盲端毛细淋巴管，第二个位于两层肌肉之间，第三个位于食管外膜。脑膜：与动脉伴行的进入颅内的集合淋巴管。淋巴管分支并在颅底孔上方触及蛛网膜。[结论]淋巴管的基本构筑是网状结构和盲端毛细血管的形成。各器官的网状结构、盲端毛细血管的长度和密度不同。

Each organ has its own architecture of lymphatic. We will present in this poster our results of the lymphatic architecture in the head and neck region using CG 3-D reconstruction method for the past three years. 【Materials and Methods】Eighteen-day fetus of mouse was extracted from the uterus and immersed in the neutral 10% formalin for fixation. After dehydration, the sample was embedded in GMA resin. Serial 3 μm sections were made and stained by toluidin-blue. Two-dimensional images of each sections input to computer by 3CCD camera and extracted lymphatic vessels and other structure by Photoshop (Adobe). These images were reconstructed to three dimensional image by Voxblast (VayTek). 【Results】 Tongue : The blind-end lymphatic capillaries entered into all connective tissue papillae at the lingual dorsum. There was the rough network and no blind-end of lymphatic capillaries at the inferior surface of the tongue. Mandible : We found the collecting lymphatic from the mandibular foramen, but not from the mental foramen. Lymphatic vessels accompanied the inferior alveolar artery, not the vein in the mandibular canal. Oesophagus : There were three lymphatic networks. First was in the lamina propria and has blind-end lymphatic capillaries, second was between two layers of muscle and third was in outer membrane of oesophagus. Meninx : The collecting lymphatic entering into the cranial cavity accompanied the artery. The lymphatic branched off and touched the arachnoid membrane just above the foramen of the cranial base. 【Conclusion】 The fundamental architecture of lymphatic vessels were the formation of the network and the blind-end capillary. The extent of network mesh, and the length and density of blind-end capillaries differ in each organs.