Architecture of lymphatic vessels on the head and neck region. -Computer graphic three dimensional reconstruction-

每个器官都有自己的淋巴结构。我们将在这张海报中展示我们在过去三年中使用CG - 3-D重建方法对头颈部区域淋巴结构的结果。【材料与方法】取小鼠子宫内18天胎儿，浸泡在10%中性福尔马林中固定。脱水后，样品包埋在GMA树脂中。连续制作3 μm切片，甲苯胺蓝染色。通过3CCD摄像机将各切片的二维图像输入计算机，用Photoshop （Adobe）软件提取淋巴管等结构。这些图像由Voxblast （VayTek）重建为三维图像。【结果】舌：盲端淋巴毛细血管进入舌背所有结缔组织乳头。舌下表面淋巴毛细血管网状粗糙，无盲区。下颌骨：我们发现了来自下颌孔的淋巴集合，但没有来自颏孔的。淋巴管伴行下牙槽动脉，而非下颌骨管静脉。食道：有三个淋巴网络。第一个在固有层有盲端淋巴毛细血管，第二个在两层肌肉之间，第三个在食道外膜。脑膜：伴动脉进入颅腔的集合淋巴。淋巴分叉并接触到颅底孔上方的蛛网膜。【结论】淋巴管的基本结构是网状结构和盲端毛细血管的形成。各个器官的网状程度、盲端毛细血管的长度和密度不同。

Each organ has its own architecture of lymphatic. We will present in this poster our results of the lymphatic architecture in the head and neck region using CG 3-D reconstruction method for the past three years. 【Materials and Methods】Eighteen-day fetus of mouse was extracted from the uterus and immersed in the neutral 10% formalin for fixation. After dehydration, the sample was embedded in GMA resin. Serial 3 μm sections were made and stained by toluidin-blue. Two-dimensional images of each sections input to computer by 3CCD camera and extracted lymphatic vessels and other structure by Photoshop (Adobe). These images were reconstructed to three dimensional image by Voxblast (VayTek). 【Results】 Tongue : The blind-end lymphatic capillaries entered into all connective tissue papillae at the lingual dorsum. There was the rough network and no blind-end of lymphatic capillaries at the inferior surface of the tongue. Mandible : We found the collecting lymphatic from the mandibular foramen, but not from the mental foramen. Lymphatic vessels accompanied the inferior alveolar artery, not the vein in the mandibular canal. Oesophagus : There were three lymphatic networks. First was in the lamina propria and has blind-end lymphatic capillaries, second was between two layers of muscle and third was in outer membrane of oesophagus. Meninx : The collecting lymphatic entering into the cranial cavity accompanied the artery. The lymphatic branched off and touched the arachnoid membrane just above the foramen of the cranial base. 【Conclusion】 The fundamental architecture of lymphatic vessels were the formation of the network and the blind-end capillary. The extent of network mesh, and the length and density of blind-end capillaries differ in each organs.