Purification and molecular basis analysis of IL-1β like substance form periodontopathic bacteria.

白介素1受体(IL-1β，IL-1β)是一种多肽类细胞因子，介导多种生理和炎症反应，是某些哺乳动物细胞的生长因子。我们发现某些牙周病细菌产生未知物质，与小鼠抗人IL-1β抗体发生反应。因此，我们检测了牙周病细菌如放线杆菌、普雷沃特氏菌、卟啉单胞菌、吞噬头孢杆菌、梭杆菌等产生IL-1β样物质的情况。酶联免疫吸附试验结果显示，伴生放线杆菌、中间普氏杆菌和黑色普氏杆菌产生IL-1β样物质。从N端的氨基酸分析表明，纯化的这些物质与人IL-1β不同。通过对RT-PCR产物的分析，也发现了差异。IL-1β样物质的抗原部位有待进一步研究。

Interleukin-1β (IL-1β) is a polypeptide cytokine that mediates many physiological to infection and inflamation and is a growth factor for certain mamalian cells. We revealed that certain periodontopathic bacteria produce unknown substance which react with mouse anti-human IL-1β antibody. Therefore, we examined the poduction of IL-1β like substance by periodontopathic bacteria, such as Actinobacills, Prevotella, Porphyromonas, Capnocytophaga, Fusobacterium. ELISA results clearly revealed that Actinobacills actinomycetemcomitans, Prevotella intermedia and Prevotella nigrescence produce IL-1β like substance. Amino acid analysis from N-terminal of affinity purified this substances were not a simillar as human IL-1β. The differensis were, also, foud form the analysis of RT-PCR products. Further investigation is required in antigenic site on IL-1β like substance.