Expression of Type I Procollagen and Amelogenin Genes in Ectoderm-Derived Mineralized Tissue

外胚层矿化组织中 I 型原胶原和牙釉蛋白基因的表达

• 批准号: 10671727
• 负责人: KOGAYA Yasutoku
• 金额: $ 1.66万
• 依托单位: Asahi University School of Dentistry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671727/
• 关键词: Enameloid; Type Collagen; Odontoblasts; Amelogenin; Hybridization; Gene Expression; Tooth Germs; Newt; エナメル質; コラーゲン; 硫酸化複合糖質; insituハイブリダイゼーション; プローブ; 石灰化

Ectoderm-derived hard tissues such as enamel, enameloid(produced jointly by epithelial and ectomesenchymal cells) and ganoine form an external highly mineralized layer on the teeth and scales respectively. The presence of collagen in enameloid distinguishes it clearly from true enamels, but little is known about phylogenetic relationship between these two tissues. The cellular source of enameloid collagen, however, has been remained controversial. With these considerations in mind we searched for collagen and amelogenin genes and characterized the origin of enameloid collagen. Messenger RNA encoding procollagen α 1 type I have been localized using digoxigenin-labelled synthetic oligonucleotide probes. The probe-mRNA hybrides were visualized using an anti-digoxin antibodies. Intense hybridization signals for the procollagen were detected in the fibroblasts of the oral mucosa connective tissues and perichondrium. Further, the positive hybridization signals were also observed in odontoblasts of the tooth germs of the larval urodele (Triturus pyrrhogaster) during enameloid matix formation. But no signals were detected in the oral mucosa epithelial cells including inner epithelial cells. It is highly suggestive that odontoblasts synthesize procollagen α 1 type I of the enameloid matrix.

外胚层衍生的硬组织，如釉质、釉质(由上皮细胞和外胚间充质细胞共同产生)和甘露碱分别在牙齿和鳞片上形成一个外部高度矿化层。牙釉质中胶原的存在明显区别于真正的牙釉质，但人们对这两种组织之间的系统发育关系知之甚少。然而，釉质胶原的细胞来源一直存在争议。考虑到这些因素，我们搜索了胶原和釉原蛋白基因，并对釉质胶原的起源进行了表征。用地高辛标记的合成寡核苷酸探针定位了编码I型前胶原α的信使RNA。用地高辛抗体对探针-mRNA杂交物进行显影。在口腔粘膜、结缔组织和软骨膜的成纤维细胞中检测到强的前胶原杂交信号。此外，在釉质基质形成过程中，还观察到了尾鱼幼虫牙胚的成牙本质细胞中的阳性杂交信号。而口腔粘膜上皮细胞包括内上皮细胞均未检测到信号。提示成牙本质细胞合成釉质基质的I型前胶原α-1。

项目成果

KOGAYA, YASUTOKU: "Immunohistochemical localisation of amelogenin-like proteins and type I colagen and histochemical demonstration of sulphated glycoconjugates in developing enameloid and enamel matrices of the larval urodele (Triturus pyrrhogaster) teeth

KOGAYA，YASUTOKU：“牙釉蛋白样蛋白和 I 型胶原蛋白的免疫组织化学定位，以及硫酸化糖缀合物在幼虫牙釉质和牙釉质基质发育中的组织化学演示

Yasutoku Kogaya: "Immunohistochemical localization of amelogenin-like proteins and type I collagen and histochemical demonstration of sulphated ……"Journal of Anatomy. 195. 455-464 (1999)

Yasutoku Kogaya：“牙釉蛋白样蛋白和 I 型胶原蛋白的免疫组织化学定位以及硫酸化的组织化学演示......”解剖学杂志 195. 455-464 (1999)

Yasutoku Kogaya: "Immunohistochemical Iocalization of dmelogenin-like proteins and type I collagen and histochemical demonstration of sulphated・・・"Journal of Anatomy. 195. 455-464 (1999)

Yasutoku Kogaya：“dmelogenin 样蛋白和 I 型胶原蛋白的免疫组织化学定位以及硫酸化的组织化学演示......”解剖学杂志 195。455-464 (1999)