The induction mechanism of new bone formation by sintered carbonate apatite grafted into bone

烧结碳酸盐磷灰石植骨诱导新骨形成的机制

• 批准号: 19592268
• 负责人: KOGAYA Yasutoku
• 金额: $ 2.16万
• 依托单位: Asahi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592268/
• 关键词: 骨; 炭酸アパタイト; 新生骨誘起; 硫酸化複合糖質; 代用骨; 炭酸含有アパタイト; 多核巨細胞; 骨形成; 電子顕微鏡

The sintered carbonate apatite grafted into rat bone at one month after implantation was coated with fibroblast-like cells, osteoblast-like cells, and/or multi-nucleated giant cells. Although part of the sintered carbonate apatite was absorbed by multinucleated giant cells or macrophages, adjacent to the surface of the survived carbonate apatite, moderate electron dense layer of about 100nm in width, was observed, where newly formed bone was frequently formed. Small type of HID-TCH-SP stain deposits that represent sulfated glycoconjugates or sulfated glycoproteins were detected over the layer.

植入大鼠骨内1个月后，烧结的碳酸磷灰石被成纤维细胞样细胞、成骨细胞样细胞和/或多核巨细胞覆盖。虽然部分烧结的碳酸磷灰石被多核巨细胞或巨噬细胞吸收，但在存活的碳酸磷灰石表面附近，观察到宽度约100 nm的中等电子致密层，其中经常形成新骨。在该层上检测到代表硫酸化糖缀合物或硫酸化糖蛋白的小类型HID-TCH-SP染色沉积物。