Experimental Study On Odontogenic Tumor Derived From Epithelial Rests Of Malassez Induced By Local Administration Of N-Methylnitrosourea(MNU)

局部给药N-甲基亚硝基脲(MNU)诱发马拉色上皮残基牙源性肿瘤的实验研究

• 批准号: 10671867
• 负责人: SUZUKI Ichiro
• 金额: $ 1.86万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671867/
• 关键词: N-emthylnitrosourea; epithelial rests of Malassez; odontogenic tumour; メチルニトロソウルア; 実験腫瘍; ラット; アルジネート; 破骨細胞; EGF

The objective of this study was to examine the possibility that epithelial rests of Malassez can give origin to odontogenic tumours. A mixture of N-methylnitrosourea (MNU) and alginate impression material for dental use was injected on the periostium of buccal side of the left mandibles of 5-week-old Wistar male rats(300mg/kg). The mixture remained at treatment-site for several months. The rats were killed 1, 3, 5, and 8 months after the injection. After 5 and 8 months, the epithelial rests of Malassez at the cervical and bifurcational portions of the first, second, and third left mandibular molars were significantly enlarged in size and the alveolar bone around the lesion was resorbed by multinucleated cells in all rats. The epithelial masses were characterized by enamel organ-like structure, deposition of eosinophilic amorphous material, duct-like structure, and squamous metaplasia. In addition to these masses in molar regions, odontogenic tumours in incisal regions and carcinomas and sarcomas in buccal region, knee, bladder, and skin were induced. Local administration of a mixture of MNU and alginate impression material for dental use can induce odontogenic tumours from the epithelial rests of Malassez in high incidence.

本研究的目的是检查马拉塞兹上皮细胞残骸可能导致牙源性肿瘤的起源。将n -甲基亚硝基脲（MNU）与牙用海藻酸盐印模材料的混合物（300mg/kg）注射于5周龄Wistar雄性大鼠左下颌骨颊侧骨膜。混合物在治疗现场保存了几个月。注射后1、3、5、8个月处死大鼠。5个月和8个月后，所有大鼠左下颌第一、第二和第三磨牙颈部和分叉部分的Malassez上皮细胞明显增大，病变周围的牙槽骨被多核细胞吸收。上皮肿块表现为珐琅质器官样结构，嗜酸性无定形物质沉积，管状结构，鳞状化生。除了磨牙区域的这些肿块外，还诱导了切牙区域的牙源性肿瘤以及颊区、膝关节、膀胱和皮肤的癌和肉瘤。局部使用MNU和海藻酸盐印模材料的混合物用于牙科，可诱导Malassez的上皮细胞产生高发病率的牙源性肿瘤。

项目成果

Hamamoto Y: "Clinical role of epithelial rests of Malassez"The Quintessense.. Vol.17. 3-12 (1998)

Hamamoto Y：“马拉塞斯上皮残骸的临床作用”The Quintessense.. Vol.17。

Hamamoto Y: "Morphological changes of epithelial rests of Malassez in rat molars induced by local administration of N-methylnitrosourea."Archs.Oral Biol.. Vol.43. 899-906

Hamamoto Y：“局部施用 N-甲基亚硝基脲诱导大鼠磨牙中马拉色上皮残基的形态变化。”Archs.Oral Biol.. Vol.43。

濱本宜興 ほか: "マラッセの上皮遺残の臨床的役割"ザ・クインテッセンス. 17巻. 3-12 (1998)

Yoshioki Hamamoto 等人：“马拉斯上皮残余物的临床作用”The Quintessence Vol. 17. 3-12 (1998)

Hamamoto,Y et al.: "Morphological changes of epithelial rests of Malassez in rat molars induced by local administration of MNU"Archives of Oral Biology. Vol.43. 899-906 (1998)

Hamamoto,Y 等人：“局部施用 MNU 诱导大鼠磨牙中马拉塞斯上皮残基的形态变化”口腔生物学档案。

Yoshioki Hamamoto: "Morphological changes of epithelial rests of Malassez in rat molors induced by local administrarion of N-methylnitrosourea"Archives of Oral Biology. 43. 899-906 (1998)

Yoshioki Hamamoto：“局部施用 N-甲基亚硝基脲诱导大鼠磨牙马拉色上皮残存的形态变化”口腔生物学档案。