Histological study of lining epithelium in odontogenic cyst with relation to differentiation, growth and immunity.

应用免疫组织化学和原位杂交技术对牙源性囊肿衬里上皮细胞分化、生长潜能和免疫反应进行了研究。免疫组化采用细胞角蛋白（CK）1、2、3、4、7、8、10、11、12、13、18、19、丝聚蛋白、外皮蛋白、EGF、EGF-γ、TGF-α、c-erbB-2、IL-1 α和IL-6的抗体，IL-1 α和IL-6原位杂交：含牙囊肿（DC）和非角化性原始囊肿（NKPC）的细胞角蛋白1，3，8，10，12，18，丝聚蛋白和外皮蛋白阴性。牙源性角化囊肿（OKC）CK 1、3、8、10、12、18、19、丝聚蛋白和外皮蛋白均为阳性。FDC和NKPC的染色模式提示衬里上皮可能具有与单纯上皮相同的特征，并可能具有独特的分化模式。OKC的角化与表皮和口腔粘膜的角化模式相同，染色不规则，不能鉴别炎性囊肿的分化和免疫反应，所有囊肿TGF-α、EGF-γ和IL-1 α均阳性，EGF、c-erbB-2和IL-6均阴性，表明衬里上皮的生长可能受TGF-β 1的调节。在所有包囊中均未检测到与所有探针的杂交信号。

Cell differentiation, growth potential and immune response in the lining epithelium of odontogenic cyst was studied with the aid of immunohistochemistry and in situ hybridization. Antibodies to cytokeratin(CK) nos.1,2,3,4,7,8,10,11,12,13,18,19, filaggrin, involucrin, EGF, EGF-gamma, TGF-alpha, c-erbB-2, IL-1alpha and IL-6 were used in immunohistochemistry and biotinilated probe of EGF-gamma, TGF-alpha, IL-1alpha and IL-6 in situ hybridization.Dentigerous cysts(DC) and non keratinizing primordial cysts(NKPC) were positive for cytokeratin nos.1,3,8,10,12,18,19 and negative for filaggrin and involucrin. Odontogenic keratocysts(OKC) were positive for CK nos.1,3,8,10,12,18,19, filaggrin and involucrin. The staining pattern of FDC and NKPC suggested that the lining epithelia may posses same character as simple epithelia and may have unique differentiation pattern. Keratinization of OKC was same pattern as epidermis and oral mucosa.Differentiation and immune response of inflammatory cyst could not be identified because of irregular staining pattern.All cysts were positive for TGF-alpha, EGF-gamma and IL-1alpha and negative for EGF, c-erbB-2 and IL-6, indicating that growth of lining epithelia may be regulated by TGF-alpha autocrine mechanism.No hybridization signal with all probes was detected in all cysts.