Histological study of lining epithelium in odontogenic cyst with relation to differentiation, growth and immunity.

牙源性囊肿内衬上皮与分化、生长和免疫相关的组织学研究。

• 批准号: 02670893
• 负责人: SUZUKI Ichiro
• 金额: $ 1.34万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670893/
• 关键词: Odontogenic cyst; Jaw cyst; Cyst epithelium; Cytokine; Interleukin; Growth factor; Cytokeratin; Terminal differentiation; インタ-ロイキン; ILー1α; ILー6; TGFーα; EGF; EGFレセプタ

Cell differentiation, growth potential and immune response in the lining epithelium of odontogenic cyst was studied with the aid of immunohistochemistry and in situ hybridization. Antibodies to cytokeratin(CK) nos.1,2,3,4,7,8,10,11,12,13,18,19, filaggrin, involucrin, EGF, EGF-gamma, TGF-alpha, c-erbB-2, IL-1alpha and IL-6 were used in immunohistochemistry and biotinilated probe of EGF-gamma, TGF-alpha, IL-1alpha and IL-6 in situ hybridization.Dentigerous cysts(DC) and non keratinizing primordial cysts(NKPC) were positive for cytokeratin nos.1,3,8,10,12,18,19 and negative for filaggrin and involucrin. Odontogenic keratocysts(OKC) were positive for CK nos.1,3,8,10,12,18,19, filaggrin and involucrin. The staining pattern of FDC and NKPC suggested that the lining epithelia may posses same character as simple epithelia and may have unique differentiation pattern. Keratinization of OKC was same pattern as epidermis and oral mucosa.Differentiation and immune response of inflammatory cyst could not be identified because of irregular staining pattern.All cysts were positive for TGF-alpha, EGF-gamma and IL-1alpha and negative for EGF, c-erbB-2 and IL-6, indicating that growth of lining epithelia may be regulated by TGF-alpha autocrine mechanism.No hybridization signal with all probes was detected in all cysts.

应用免疫组织化学和原位杂交技术对牙源性囊肿衬里上皮的细胞分化、生长潜能和免疫反应进行了研究。用细胞角蛋白(CK)1、2、3、4、7、8、10、11、12、13、18、19、丝状蛋白、总蛋白、EGF-γ、转化生长因子-α、c-erbB-2、IL-1α、IL-6的抗体进行免疫组织化学染色及EGF-γ、转化生长因子-α、IL-1α、IL-6的原位杂交。牙源性角化囊(OKC)CK 1、3、8、10、12、18、19、微丝蛋白和总蛋白均阳性。FDC和NKPC的染色模式提示衬里上皮可能具有与单纯上皮相同的特征，并可能具有独特的分化模式。OKC的角化类型与表皮和口腔粘膜相同，炎性囊变的分化和免疫反应因染色不规则而无法识别，所有囊泡均为阳性，EGF、c-erbB-2和IL-6均为阴性，提示衬里上皮的生长可能受转化生长因子-α自分泌机制的调控，所有囊泡均未检测到与所有探针杂交信号。