Study on Apoptotic cell death and mechanism of metastasis of adenoid cystic carcinoma derived cell line.

腺样囊性癌来源细胞凋亡及转移机制的研究。

• 批准号: 10671876
• 负责人: RYOKE Kazuo
• 金额: $ 2.05万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671876/
• 关键词: ACC-3; Apoptosis; 5-Fluorouracil; Nedaplation; Carboplation; p53; VEGF; 5ーFluorouracil; P53

We investigated on cell death in cultured human adenoid cystic carcinoma cell line (ACC-3) induced by anti-cancer drugs of 5-Fluorouracil (5-Fu), Nedaplatin (Ned) and Carboplatin (CBDCA), added to apoptosis of such cell line.We also investigated on squamous cell carcinoma such as HSC-2, HSC-3, HSC-4 and Ca9-22 in addition to ACC-3.We investigated growth-inhibitory effect of 5-Fu was the most prominent in Ca 9-22, followed by ACC-3 and HSC-4, but no any effects were found in HSC-2 and HSC-3. As for Ned, the growth-inhibitory effects were obtained in all cell lines tested, the most prominent in HSC-3, followed by HSC-4, Ca9-22, HSC-2 and ACC-3(55%). The growth-inhibitory effect of CBDCA was the most prominent in HSC-2, followed by HSC-4, HSC-3 and Ca9-22(60%).By TUNEL method apoptosis of tumor cell lines were estimated with apoptotic index (AI). AI of HSC-3, HSC-2, HSC-4 and ACC-3 with 5-Fu after 24 hours were 12%, 10%, 8% and 2% respectively.As for Ned the AI of HSC-3, HSC-4 and ACC-3 were 3%, 9% and 6%, respectively. As for CBDCA AI of ACC-3 was 3%.Induction mechanism of apoptosis was intended to be obvious with confirming effect individually in each of 5-Fu, Ned and CBDCA was ascertained, so relation to distant metestasis was investigated with examining of VEGF in addition to p53. As a result, relationship of p53 and VEGF was distinct.

本实验研究了抗癌药物5-氟尿嘧啶（5-Fu）、奈达铂（Ned）和卡铂（CBDCA）对体外培养的人腺样囊性癌细胞系ACC-3的诱导凋亡作用，以及对鳞状细胞癌HSC-2、HSC-3、5-Fu对Ca 9 - 22细胞的生长抑制作用最强，其次是ACC-3和HSC-4，而对HSC-2和HSC-3细胞的生长抑制作用不明显。对于Ned，在所有测试的细胞系中获得生长抑制作用，在HSC-3中最突出，其次是HSC-4、Ca 9 -22、HSC-2和ACC-3（55%）。CBDCA对HSC-2细胞的生长抑制作用最明显，其次为HSC-4、HSC-3和Ca 9 -22（60%）。5-Fu对HSC-3、HSC-2、HSC-4和ACC-3的抑制率分别为12%、10%、8%和2%，对Ned的抑制率分别为3%、9%和6%。ACC-3对CBDCA的AI为3%，5-Fu、Ned和CBDCA的诱导凋亡机制是明显的，因此除了p53外，还通过检测VEGF来研究其与远处转移的关系。p53与VEGF的表达有明显的相关性。

项目成果

Reiko Doi: "Expression of p53 oncoprotein increases inratumoral microvessel formation in human salivary gland carcinomas"J. Oral Pathol. Med.. 28. 259-263 (1999)

Reiko Doi：“p53 癌蛋白的表达增加了人类唾液腺癌中瘤内微血管的形成”J。

RIEKO Doi: "Expression of p53 oncoprotein increases intratumoral microvessel formation in human salivary gland carcinomas"J. Oral. Pathol Med.. vol.28. 259-263 (1999)

RIEKO Doi：“p53 癌蛋白的表达增加了人类唾液腺癌中瘤内微血管的形成”J。

Rieko Doi: "Expression of p53 oncoprotein increases inratumoral microvessel formation in human salivary gland Carcinomas"J.Oral Pathol Med. 28. 259-263 (1999)

Rieko Doi：“p53 癌蛋白的表达增加了人类唾液腺癌中瘤内微血管的形成”J.Oral Pathol Med。