Resolving orofacial neuropathic pain evoked by compression of a trigeminal nerve branch using rationally integrated complementary approaches

使用合理整合的补充方法解决三叉神经分支受压引起的口面部神经性疼痛

• 批准号: 9703533
• 负责人: WOLFGANG B. LIEDTKE
• 金额: $ 30.51万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-20 至 2021-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9703533
• 关键词: Affect; Affective; Afferent Neurons; American; Behavior; Biological Assay; Blood Cells; Body Regions; Brain; Brain Stem; Calcium; Chronic; Complement; Complementary and alternative medicine; Cornea; Data; Dinoprostone; Disease; Effectiveness; Electroacupuncture; Emotional; Enzyme-Linked Immunosorbent Assay; Female; Goals; Human; Hypersensitivity; Image; Implant; Incidence; Inflammation; Inflammatory; Intake; Lateral; Light; Measures; Meninges; Mus; Nerve; Nerve Pain; Neuroglia; Neuronal Plasticity; Neurons; Organ; Pain; Pathway interactions; Patients; Peripheral nerve injury; Persistent pain; Plant Roots; Population; Quality of life; Resolution; Self-Injurious Behavior; Sensory; Serum; Sinus; Site; Stress; Structure; Structure of trigeminal ganglion; Structure of trigeminal nerve spinal tract nucleus; Swimming; Temporomandibular Joint; Testing; Time; Tooth structure; Trigeminal Pain; Trigeminal System; Trigeminal nerve structure; Tumor-infiltrating immune cells; Victimization; Work; base; behavior measurement; behavioral response; chemokine; chronic neuropathic pain; chronic pain; clinical practice; constriction; craniofacial; cytokine; effectiveness testing; experimental study; improved; in vivo; lipid mediator; macrophage; mouse model; nerve injury; neural circuit; neuroinflammation; non-opioid analgesic; orofacial; painful neuropathy; parabrachial nucleus; response; satellite cell; sensory stimulus; sex; stem; vocalization

Project 1: Resolving orofacial neuropathic pain evoked by compression of a trigeminal nerve branch using rationally integrated complementary approaches ABSTRACT Neuropathic pain in the craniofacial region innervated by trigeminal sensory neurons affects approximately at least 1x106 Americans. The goal of this project is to assess efficiency and elucidate key mechanisms of rationally-combined complementary approaches to treat trigeminal neuropathic pain. The central hypothesis of Project-1 is that natural pro-resolution lipid mediators (specialized pro-resolution lipid mediators, SPMs) or auricular electro-acupuncture (aEA) can produce beneficial effects in alleviating orofacial neuropathic pain, aggravated by stress, by modulating neuroinflammation and neural plasticity. We will use a well-established mouse model of trigeminal neuropathic pain, chronic constriction of the infraorbital nerve (CCI-ION), to test our central hypothesis. We will first define the time course of neuropathic pain and inflammatory and neuroinflammatory profiles in response to CCI-ION in both sexes with and without stress. Subsequently, we will determine the effects of aEA and SPMs on pain-related behaviors as well as resolution of inflammatory and neuroinflammatory profiles evoked by CCI-ION. Finally, we will explore effectiveness of aEA and SPMs on reducing spontaneous activity of trigeminal sensory neurons and of ongoing pain-activated CNS neurons after CCI-ION.

项目1：解决三叉神经分支压迫引起的口面神经病理性疼痛 使用合理整合的互补方法 摘要 三叉神经感觉神经元支配的颅面区域的神经病理性疼痛大约影响 1x 106美国人该项目的目标是评估效率和阐明的关键机制， 合理组合的互补方法来治疗三叉神经痛。核心假设 项目-1的一个重要方面是天然促消退脂质介质（专门促消退脂质介质，SPM）或 耳穴电针（aEA）可以在减轻口面神经病理性疼痛方面产生有益的作用， 通过调节神经炎症和神经可塑性而加重。我们将使用一个完善的 三叉神经病理性疼痛的小鼠模型，眶下神经慢性收缩（CCI-ION），以测试 我们的核心假设我们将首先定义神经性疼痛和炎症的时间过程， 在有和无应激的两种性别中，CCI-ION对神经炎症的反应。后续我们 将确定aEA和SPM对疼痛相关行为以及炎症反应的影响。 和CCI-ION诱发的神经炎症特征。最后，我们将探讨aEA和SPM的有效性 减少三叉神经感觉神经元和持续疼痛激活的CNS神经元的自发活动 CCI-ION之后。