EFFECT OF PARTIAL DISC-REMOVAL ON THE INTRA-ARTICULAR ARCHITECTURE OF THE SHEEPTMJ.

部分椎间盘切除对羊 TMJ 关节内结构的影响。

• 批准号: 10671914
• 负责人: OGI Nobumi
• 金额: $ 1.15万
• 依托单位: AICHI-GAKUIN UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671914/
• 关键词: TMJ; disc perforation; remodeling; synovial fluid; 関節円板; グリコサミノグリカン; ヒアルロン酸; 羊

The disc is an essential part of the complicated biomechanics of the TMJ.Normal TMJ function is maintained with the disc. Previous studies reported that experimental disc perforation or surgically induced disc displacement affects the shape of the TMJ.Altered disc position clinically leads to the condylar changes in shape and size. However, little information is known about the changes. Whether disc perforation/absence affects joint morphology is ambiguous. The purpose of this study is to investigate the effect of experimental partial disc-removal on the intra-articular architecture of the sheep TMJ.The following two procedures were carried out for the sheep TMJ.Group 1 : The lateral half of the disc was removed in the bilateral sheep TMJ.Group 2 : Lateral half of the disc was cut comb-like in the bilateral sheep TMJ.The animals were sacrificed at 2, 4, 12, and 24 weeks after the partial disc removal. The joint was removed 'en bloc', histologically processed and examined. The synovial fluid was collected before and do the procedure. Chondroitin-sulfate levels of the synovial fluid were measured by HPLC analysis.Morphologic, histologic, and biochemical analysis showed almost consistent results in the Group 1 and Group 2. The morphologic changes were notably observed at the lateral part of the condyle, corresponding to the area of disc-removal/comb-like cut. The lateral condylar articular cartilage proliferated and increased in its thickness with time. Synovial fluid chondroitin-sulfate levels increased at 2 and 4 postoperative weeks in the both groups, but were not significantly different to the pre-operative values.These changes after disc-removal were identical to cartilagenous proliferative remodeling rather than osteoarthritis. The condylar morphologic changes were attributed to proliferative remodeling and probably due to increased mechanical loading.

椎间盘是TMJ复杂生物力学的重要组成部分。正常的颞下颌关节功能与椎间盘保持一致。先前的研究报道了实验性椎间盘穿孔或手术诱导的椎间盘移位会影响TMJ的形状。临床上椎间盘位置的改变导致髁突形状和大小的改变。然而，人们对这些变化知之甚少。椎间盘穿孔/缺失是否影响关节形态尚不明确。本研究的目的是探讨实验性部分椎间盘切除对绵羊TMJ关节内结构的影响。对绵羊颞下颌关节进行了以下两项手术。第一组：切除双侧羊TMJ椎间盘的外侧半盘。第二组：双侧羊TMJ椎间盘外侧半侧呈梳状切开。在椎间盘部分切除后2周、4周、12周和24周处死动物。将关节“整体”切除，进行组织学处理和检查。在手术前收集滑液。采用高效液相色谱法测定滑液硫酸软骨素水平。形态学、组织学和生化分析显示1组和2组的结果几乎一致。在髁外侧观察到明显的形态学改变，对应于椎间盘移除/梳状切口的区域。外髁关节软骨增生，厚度随时间增加。两组术后2周和4周滑膜液硫酸软骨素水平均升高，但与术前无显著差异。椎间盘去除后的这些变化与软骨增生性重塑相同，而不是骨关节炎。髁突形态的改变归因于增生性重塑，可能是由于机械负荷的增加。