The relationship between diabetes mellitus and experimental periodontitis in assaciation with neurogenic inflammation

取Wistar大鼠100只，自发性糖尿病（GK）大鼠100只，分为4组；J-1：非应激Wistar大鼠，J-2：抑制应激Wistar大鼠，G-1：非应激GK大鼠，G-2：抑制应激GK大鼠。在上颌右侧第二磨牙周围结扎。应激组（J-2和G-2）在10天内连续12小时用钢丝网进行应激处理。每组10只大鼠于第2、4、6、8、10天处死。献祭时取外周血，切除胸腺和上颌骨。上颌依次固定、脱钙、嵌套、切片。对上颌第二磨牙分岔区进行组织学和组织学检查。应激组大鼠体重明显低于非应激组（J-1和G-1）。应激组血糖水平明显升高。血浆促肾上腺皮质激素和肾上腺素浓度J-2明显高于J-1。另一方面，G-2中相同浓度在第2、4天显著高于G-1，但在第6天后显著降低。应激组胸腺明显萎缩。组织学上观察到在J-2分叉区神经纤维上有大量绒毛，可能提示神经源性炎症。但G-2没有这样的发现。应激组第8、10天的牙槽骨吸收显著高于非应激组。本研究结果提示约束应力可能是实验性牙周炎的危险因素之一。然而，糖尿病大鼠在应激条件下对牙周组织的影响机制可能与正常大鼠不同。

One hundred Wistar rats and 100 spontaneous diabetes mellitus (GK) rats were included in this study, and divided into 4 groups ; J-1 : non-stress Wistar rats, J-2 : restraint-stress Wistar rats, G-1 : non-stress GK rats, G-2 : restrain-stress GK rats. A ligation was done around the 2nd molar in the maxillary right sides. The rats in the stress group (J-2 and G-2) exposed to a stressful condition by the use of wire mesh for 12 hours during 10 days. Ten rats in each group were sacrificed on days 2, 4, 6, 8, and 10. At the time of sacrifice, the peripheral blood was taken and the thymus and the maxillae were removed. The maxillae were fixed, decalcified, embedded, and sectioned serially. The furcation area of the 2nd maxillary molars were examined histologically and histometrically.The body weights in the stress group were reduced than those of the non-stress group(J-1 and G-1). The fasten blood glucose in the stress group were significantly increased. The concentrations of plasma adrenocorticotropic hormone and adrenaline in J-2 were significantly increased than those of J-1. On the other hand, the same concentrations in G-2 were significantly increased than those of G-1 on days 2, 4, however, significantly reduced after the 6th day. The thymus in the stress group was significantly atrophied. Numerous tufts on nerve fibers that may indicate a neurogenic inflammation were observed in the furcation area of J-2 histologically. But there were not such findings in G-2. The alveolar bone resorption in the stress group on days 8, and 10 were significantly greater than that of non-stress group histometrically. The results of this study indicated that the restraint stress may be one of the risk factors for experimental periodontitis. However, in diabetic rats, the mechanism affects the periodontium under the stressful condition may differ from normal rats.