Experimental Periapical Lesion in Non-insulin Dependent Diabetic Rat (GK Rat).

非胰岛素依赖性糖尿病大鼠（GK 大鼠）的实验性根尖周损伤。

• 批准号: 09671973
• 负责人: NAKAMURA Hiroshi
• 金额: $ 1.98万
• 依托单位: Dependent of Endodontics, School of Dentistry, Aichi Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671973/
• 关键词: GK rat; Periapical Lesion; Non-insulin Dependent Diabetes; 糖尿病; GK Rat; 病理組織学

The purpose of the present study was to investigate histologically and histometrically the effect of a sucrose solution on the periapical lesion of spontaneous non-insulin dependent diabetes mellitus rats (GK Rat) which were made by Goto ＆ Kakizaki. Twenty GK rats and 20 JCL Wistar rats (JCL Rat) were used in this study. All animals received a pulpal exposure treatment in the left mandibular first molar. Then, the animals were divided equally into two groups of JCL rats (J-1, J-2) and two groups of GK rats (G-1, G-2). J-1 and G-1 received a normal laboratory diet and water, while J-2 and G-2 received a normal laboratory diet and 30% sucrose solution. The animals of all 4 groups were sacrificed 2 and 4 weeks after pulpal exposure for histological observation. The blood glucose tests showed higher values at 4 and 12 weeks in G-1 and G-2 than in J-1 and J-2. Pulpal tissues (PT) and periapical tissues (PAT) of the mandibular first molar were examined histologically. Moreover, the area of the periapical lesion in PAT was measured histometrically. Histologically, the alveolar bone resorption in PAT was severer in J-2 than that in G-2 throughout the experimental period. The histometrical study revealed that the area of the periapical lesion was significantly larger in G-2 at 2 and 4 weeks than that in J-2 (p<0.01). The results of the present study indicate that the metabolic condition of non-insulin dependent diabetes mellitus may enhance the development of periapical lesions in rats.

本研究的目的是从组织学和组织计量学的角度探讨蔗糖溶液对后藤& Kakizaki等制作的自发性非胰岛素依赖型糖尿病大鼠（GK Rat）根尖周病变的影响。本研究使用20只GK大鼠和20只JCL Wistar大鼠（JCL Rat）。所有动物在左下颌第一磨牙中接受牙髓暴露治疗。然后，将动物等分为两组JCL大鼠（J-1，J-2）和两组GK大鼠（G-1，G-2）。J-1和G-1接受正常实验室饮食和水，而J-2和G-2接受正常实验室饮食和30%蔗糖溶液。分别于牙髓暴露后2周和4周处死动物进行组织学观察。G-1和G-2的血糖测试显示在第4周和第12周的值高于J-1和J-2。对下颌第一磨牙的牙髓组织（PT）和根尖周组织（PAT）进行组织学观察。此外，组织学测量PAT中根尖周病变的面积。组织学上，在整个实验期间，PAT中的牙槽骨吸收在J-2中比在G-2中更严重。组织计量学研究显示，在第2周和第4周，G-2组的根尖周病变面积明显大于J-2组（P<0.01）。本研究结果提示，非胰岛素依赖型糖尿病的代谢状态可促进大鼠根尖周病变的发生。