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STRUCTURE AND FUNCTION OF ANIMAL ION TRANSPORTER THAT EXPRESS IN BACTERIA

STRUCTURE AND FUNCTION OF ANIMAL ION TRANSPORTER THAT EXPRESS IN BACTERIA
细菌中表达的动物离子转运蛋白的结构和功能
批准号:
10672037
负责人:
NAKAMURA Tatsunosuke
金额:
$1.6万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1998
资助国家:
日本
项目状态:
已结题
起止时间:
1998 至 2000

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中文摘要
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英文摘要
TRP (transient receptor rotential) homologue of mouse function as cation channel that passes Ca^<2+> and Na^+. When TRP homologue gene was transformed in Escherichia coli mutant strain TO114 which lacks all Na^+/H^+ antiporters, this strain became sensitive to Na^+ suggesting that it functions in E.coli membrane. We detected His tagged TRP homologue expressed in membrane fraction of E.coli. TRP probably has common structure with VR1, capsaicin receptor, but those structures are not known. TRP/VR1 were suggested to have homology with Shaker type K^+ channels with 6 transmembrane regions, though there are no experimental reports. We started experiment for TRP structure using bacterial system and got some results. We are doing further experiment using mammalian cultured cells to confirm the TRP structure that got from bacterial system.
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会议论文
A.Hamada: "The Arabidopsis HKT1 Gene Homolog Mediates Inward Na^+ Currents in Xenopus laevis Oocytes and Na^+ Uptake in Saccharomyces cerevisiae."Plant Physiology. 125. 437-446 (2001)
A.Hamada:“拟南芥 HKT1 基因同源物介导非洲爪蟾卵母细胞的内向 Na^ 电流和酿酒酵母的 Na^ 吸收。”植物生理学。
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通讯作者:
Y.Nakamaru, Y.Takahashi, T.Unemoto, T.Nakamura.: "Mechanosensitive channel (MscL) functions to alleviate the cell lysis of marine bacterium, Vibrio alginolyt."FEBS Letters. 444. 170-172 (1999)
Y.Nakamaru、Y.Takahashi、T.Unemoto、T.Nakamura.:“机械敏感通道 (MscL) 的功能是减轻海洋细菌溶藻弧菌的细胞裂解。”FEBS 快报。
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N.Uozumi: "The Arabidopsis HKT1 Gene Homolog Mediates Inward Na^+ Currents in Xenopus laevis Oocytes and Na^+ Uptake in Saccharomyces cerevisiae."Plant Physiology. 122. 1249-1260 (2000)
N.Uozumi:“拟南芥 HKT1 基因同源物介导非洲爪蟾卵母细胞的内向 Na^ 电流和酿酒酵母的 Na^ 吸收。”植物生理学。
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通讯作者:
T.Nakamura, N.Yamamuro, S.Stumpe, T.Unemoto, E.P.Bakker.: "Cloning of the trkAH gene cluster and characterization of the Trk K^+-uptake system of Vibrio alginoyticus."Microbiology. 144. 2281-2289 (1998)
T.Nakamura、N.Yamamuro、S.Stumpe、T.Unemoto、E.P.Bakker.:“藻酸弧菌 trkAH 基因簇的克隆和 Trk K^-摄取系统的表征。”微生物学。
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32
    Research for structure and function of bacterial cation transport systems
    Research for function of antiporters and devices for membrane biogenesis using bacteria.
    Genes those regulate internal osmolality
    • 批准号:
      07672344
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $1.02万
    • 财政年份:
      1995
    • 负责人:
      NAKAMURA Tatsunosuke
    • 依托单位:
    海外基金