Genes those regulate internal osmolality

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(1) trkAv and neighboring genes.We clone K^+ uptake genes, trkAv and trkHv, from marine bacterium, Vibrio alginolyticus, those functionally complement K^+ uptake deficient E.coli strains. K^+ uptake activities of those strains with trkAv and/or trkHv genes are determined. Expression of TrkAv and TrkHv were examined also.(2) mscL gene.We examined the activity of MscL in Vibrio alginolyticus.(3) nhaBv gene.We cloned nhaBv, the second Na^+/H^+ antiporter, gene from V.alginolyticus. Topological experiments were done by phoA gene fusion method. It was appeared that NhaBv has odd number transmembrane segments.