Genes those regulate internal osmolality

调节内部渗透压的基因

• 批准号: 07672344
• 负责人: NAKAMURA Tatsunosuke
• 金额: $ 1.02万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672344/
• 关键词: trkA; mscL; nhaB; osmolality; gene; proC

(1) trkAv and neighboring genes.We clone K^+ uptake genes, trkAv and trkHv, from marine bacterium, Vibrio alginolyticus, those functionally complement K^+ uptake deficient E.coli strains. K^+ uptake activities of those strains with trkAv and/or trkHv genes are determined. Expression of TrkAv and TrkHv were examined also.(2) mscL gene.We examined the activity of MscL in Vibrio alginolyticus.(3) nhaBv gene.We cloned nhaBv, the second Na^+/H^+ antiporter, gene from V.alginolyticus. Topological experiments were done by phoA gene fusion method. It was appeared that NhaBv has odd number transmembrane segments.

(1)我们从海洋细菌溶藻弧菌（Vibrio alginolyticus）中克隆了K^+摄取基因trkAv和trkHv，它们在功能上补充了K^+摄取缺陷的大肠杆菌菌株。测定了带有trkAv和/或trkHv基因的菌株的K^+吸收活性。还检测了TrkAv和TrkHv的表达。(2)我们检测了溶藻弧菌中MscL的活性。(3)nhaBv基因：我们从溶藻弧菌中克隆了第二种Na^+/H^+反向转运蛋白nhaBv基因。采用phoA基因融合方法进行拓扑实验。NhaBv似乎具有奇数个跨膜片段。

项目成果

Tatsunoske Nakamura et al.: "Cloning and sequencing of novel genes from Vibrio alginolyticus" Biochemica et Biophysica Acta.vol 1277. 201-208 (1996)

Tatsunoske Nakamura 等：“来自溶藻弧菌的新基因的克隆和测序”Biochemica et Biophysicala Acta.vol 1277. 201-208 (1996)

Tatsunosuke Nakamura et al.: "Cloning and seqnencing of novelgenes from Vibrio alginolyticns" Biochemica et Biophysica Acta. 1277. 201-208 (1996)

Tatsunosuke Nakamura 等人：“溶藻弧菌新基因的克隆和测序”《生物化学与生物物理学学报》。

Tatsunosuke Nakamura et al.: "Cloning and seqnencing of the nhaB gene encoding an Na^+/H^+ antipester." Biochemica et Biophysica Acta. 1275. 157-160 (1996)

Tatsunosuke Nakamura 等人：“编码 Na^ /H^ 抗虫剂的 nhaB 基因的克隆和测序。”

Tatsunosuke Nakamura et al.: "Cloning and sequencing of the nhaB gene encoding an Na^+/H^+ antiporter." Biochemica et Biophysica Acta.vol 1275. 201-208 (1996)

Tatsunosuke Nakamura 等人：“编码 Na^ /H^ 反向转运蛋白的 nhaB 基因的克隆和测序。”

Tatsunosuke Nakamura et al.: "Cloning and sequencing of novel genes from Vibrio alginolyticus" Biochimica et Biophysica Acta. 1277. 201-208 (1996)

Tatsunosuke Nakamura 等人：“溶藻弧菌新基因的克隆和测序”Biochimica et Biophysicala Acta。