Effect of high fat diet on endurance capacity and oxydative enzyme activities in rat skeletal muscle.

为探讨高脂饮食对大鼠耐力和骨骼肌氧化酶活性的影响，将雄性Wistar大鼠分为高脂饮食组和低脂饮食组。他们随意吃这些饮食5周。虽然高脂饮食组的三头肌糖原浓度约为低脂饮食组的一半，但高脂饮食组的游泳能力显著高于低脂饮食组。高脂饮食诱导三头肌中柠檬酸合酶（CS）和3-羟酰辅酶A脱氢酶（3-HAD）活性显著增加（分别为23%和47%）。Wistar大鼠被喂食四种饮食之一：低脂（12%卡路里作为脂肪）、猪油（60%卡路里作为脂肪）、红花油（60%卡路里作为脂肪）或鱼油（60%卡路里作为脂肪）。各饲料组间体重无显著差异。猪油组和红花油组的表皮脂肪组织重量高于低脂组。结果表明，长期高脂饮食可使骨骼肌红部CS和3-HAD活性显著升高，提示高脂饮食可使骨骼肌线粒体酶活性升高，从而促进糖原节约，提高耐力。

The purpose of this study was to determine the effect of high fat diet on endurance capacity and oxidative enzyme activities in rat skeletal muscle.Male Wistar rats were assigned to either a high fat diet and a low fat diet group. They ate these diets ad libitum for 5 weeks. Although triceps muscle glycogen concentration in the high fat diet group was about half of that in the low fat diet group, swimming capacity was significantly higher in the high fat than in the low fat diet group. The high fat diet induced significant increases in citrate synthase (CS) and 3-hydroxyacyl CoA dehydrogenase (3-HAD) activities in triceps muscle (23% and 47%, respectively).Wistar rats were fed one of four diets : low fat (12% calories as fat) , lard (60% calories as fat) , safflower oil (60% calories as fat) , or fish oil (60% calories as fat). There were no significant differences in body weight among each diet group. Epidydimal adipose tissue weight was higher in lard and safflower oil groups than in low fat. Safflower and fish oil diet induced significant increases in CS and 3-HAD activities in red portion of gastrocnemius muscle.These results suggest that long term feeding of a high fat diet increases mitochondrial enzyme activities in skeletal muscle, and these increases might induce glycogen sparing and thus improve endurance.