Effect of different type of high fat diets on glycogen loading

不同类型高脂饮食对糖原负荷的影响

• 批准号: 13680028
• 负责人: NAKATANI Akira
• 金额: $ 2.18万
• 依托单位: Nara University of Education
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680028/
• 关键词: glycogen; 3-HAD; high fat diet; rat; middle chain fatty acid; unsaturated fatty acid; GLUT4; glycogen loading; グリコーゲン合成酵素

The purpose of this study was to determine the effect of high fat diet on glycogen loading in rats, Male Wistar rats were assigned to low fat diet (13% calories as fat) and a high fat diet (60% calories as fat). They ate their diets ad libitum for 5 weeks, After an overnight fast, both groups were exercised by using a two hours swimming protocol to deplete muscle glycogen. After the swimming, rats were given a high carbohydrate diet and 5% sucrose solution ad libitum. Rats were anesthetized with pentobarbital sodium, and the triceps and vastus lateralis muscles were dissected out before, immediately after, 4h after and 24h after exercise. Epididymal fat pad weight, plasma triglyceride and free fatty acids were significantly (P<0.001) higher in the high fat diet group than in the low fat group, Although the high fat diet decreased muscle glycogen concentration before exercise, the high fat diet induced increases in citrate synthase and 3-hydroxyacyl CoA dehydogenase activities, Glycogen concentration in both groups were depleted immediately after the exercise and recovered to normal level 24h after exercise. These results suggest that long term feeding of high fat diet harm the effect of glycogen loading.

本研究的目的是确定高脂肪饮食对大鼠糖原负荷的影响。雄性Wistar大鼠被分配到低脂肪饮食（13%卡路里作为脂肪）和高脂肪饮食（60%卡路里作为脂肪）。他们自由进食5周，禁食一夜后，两组都进行了两小时的游泳运动，以消耗肌糖原。游泳后，大鼠自由进食高碳水化合物饲料和5%蔗糖溶液。大鼠在戊巴比妥钠麻醉下，于运动前、运动后即刻、运动后4h和运动后24h分别取肱三头肌和股外侧肌。附睾脂肪垫重量、血浆甘油三酯和游离脂肪酸（P <0.001）高脂饮食组运动前肌糖原含量显著低于低脂饮食组，但高脂饮食组运动前肌糖原含量显著低于低脂饮食组，运动前高脂饮食组运动后肌糖原含量显著高于低脂饮食组，运动后高脂饮食组运动前肌糖原含量显著低于低脂饮食组，运动后高脂饮食组运动前肌糖原含量显著高于低脂饮食组，运动后高脂饮食组运动前肌糖原含量显著高于低脂饮食组，运动后高脂饮食组运动前肌糖原含量显著高于低脂饮食组，运动后高脂饮食组运动前肌糖原含量显著高于低脂饮食组，运动后高脂饮食组运动后肌糖原含量显著高于低脂饮食组，运动后高脂饮食组运动前肌糖原含量显著高于低脂饮食组，运动后高脂饮食组运动后肌糖原含量显著高于低脂饮食组，运动后高脂饮食组运动前肌糖原含量显著高于低脂饮食组，运动后高脂饮食组运动后肌糖原含量显著高于低脂饮食组，运动后高脂饮食组运动前肌糖原含量显著高于低脂饮食组，运动后高脂饮食组两组运动后糖原浓度均迅速下降，运动后24 h恢复正常。提示长期高脂饲料喂养损害糖原负荷的效果。