Anti-platelet aggregation and plasma fibrinolysis-accelerating activities in oyster fungus (Pleurotus ostreatus)

平菇(Pleurotus Ostreatus)对ADP、胶原、凝血酶诱导的人血小板聚集有较强的阻断作用(220×106/ml)。在pH 4.5条件下，曲霉/根霉酶处理24小时，活性物质(ASK-1)可以很容易地用水提取。在10 0℃下加热5min停止反应，膜过滤和冷冻干燥后，从1.7kg蘑菇中获得约2 7g的暗黄色粉末。用人PRP和ADP计算的IC50约为11.6-27.1 mg/ml。经口给药，ASK-1对大鼠和人血中的血小板聚集均有较强的抑制作用。6名18~37岁健康志愿者口服5 0 0 mg/kg体重时，以10μ二磷酸腺苷为诱导剂，血小板聚集率由65±8%降至50±13%。而EFA和tPA测定的纤溶活性则升高。酶谱结合纤溶促进物(Fas)分析表明，人细胞释放的活性纤溶酶为tPA型纤溶酶原激活剂，其相对分子质量约为7万。亚硝酸盐法测得958±402CU-SOD活性/g(干重)，高于文献报道的纳豆。用Celite545柱分离得到的浓缩组分对2-脱氧核糖也有一定的抗氧化活性。在健康的人类志愿者中摄入该组分后，证明对去雄和减肥都有很强的抑制作用。

Oyster fungus (Pleurotus ostreatus) had strong activity for blocking of the aggregation of human platelets (220 × 10^6/ml) caused by ADP, collagen or thrombin. Active substance (named ASK-1) treated with Aspergillus/Rhizopus enzyme for 24 hrs at pH 4.5 could be easely extracted with water. After stopping the reaction by heating at 100 ℃ for 5 min, and then membrane filtering and lyophilizing, approximately 27 g of dark yellow powder were obtained from 1.7 kg mushroom. The IC_<50> value calculated with human PRP and ADP for the inducer was about 11.6-27.1 mg/ml.By oral administration, ASK-1 showed strong inhibition for platelet aggregation in blood of both rats and humans. When administerd 500 mg/kg body in 6 healthy volunteers of 18-37 years, the platelet aggregation was decreased from 65±8% to 50± 13% using 10 μ M ADP for the inducer. Whereas the fibrinolytic activity determined by EFA and tPA were increased. By using zymography coupled with fibrinolysis accelerating substance (FAS), the activated fibrinolytic enzyme released from human cell was found to be tPA type plasminogen activator with molecular weight of about 70,000.Oyster fungus had strong SOD and anti-oxidative activities. Values of 958±402 CU SOD activity/g (dry weight) determined by nitrite method was higher than that of Natto reported previously. The concentrated fraction using Celite 545 column had also anti-oxidative activity against 2-deoxylibose. After ingestion of this fraction in healthy human volunteers, both strong inhibitions for apetite and loss of weight were proved.