Dynamic analysis of coliform group in the sea and estuary of Ariake-kai bay

有明湾海域及河口大肠菌群动态分析

• 批准号: 10680505
• 负责人: ISHIDA Akio
• 金额: $ 1.6万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680505/
• 关键词: Ariake-kai; Escherichia coli; coliform group; survival; sea; river; tideland; coastal zone

Bacteria of coliform group concluding Escherichia coli are not usually found in natural environment such as sea and river. However, it is known that such bacteria can be detected in the sea and river polluted by sewage of towns and cities. In present study, we attempt to clarify the dynamic analysis of coliform group in the sea and estuary of Ariake-kai bay. In Shirakawa River, that flows in Ariake-kai through Kumamoto city, we observed a large amount of coliform group. And, we could detect also the bacteria in the whole sea area of Ariake-kai bay, though the amount of those was a little. From the experimental observation that cells of Escherichia coli could survive in seawater for at least one month, it is considered that a constant amount of coliform group may exist always in the sea and coastal zone of Ariake-kai.However, we found the interesting fact that coliform group could not detect in several tidelands of Ariake-kai. From these results, some factor(s) in the soil of tideland are considered to affect the survival of coliform group. Therefore, we carried out to identify the factor(s) in the soil of that. Although we could not identify perfectly the factor(s), it seems that the factor(s) are certain microorganism(s) which are lethal over the temperature of 60 ℃ and their size of cells is l.2〜5.0μm. Further study is needed on the identification and property of the factor(s) of tideland. It should be very interesting to clarify the role of tideland affecting the preservation of natural environment contaminated by external bacteria such as Escherichia coli. Studies along this line are in progress in our laboratory.

以大肠杆菌为代表的大肠菌群细菌通常在海洋和河流等自然环境中不存在。然而，众所周知，在被城镇污水污染的海洋和河流中可以检测到这种细菌。在本研究中，我们试图阐明有明湾海域和河口大肠菌群的动态分析。在流经熊本市的有明海的白川中，观察到了大量的大肠菌群。而且，在有明湾整个海域也能检测到细菌，虽然数量很少。从实验观察到大肠杆菌细胞可以在海水中存活至少一个月，因此认为有明海的海域和海岸带可能始终存在恒定量的大肠菌群。然而，我们发现了一个有趣的事实，在有明海的几个滩涂中无法检测到大肠菌群。从这些结果来看，滩涂土壤中的某些因素被认为影响大肠菌群的生存。因此，我们对其土壤中的因素进行了鉴定。虽然我们不能完全确定这些因素，但似乎这些因素是某些在60℃以上具有致死性的微生物，其细胞大小为l.2〜5.0μm。滩涂因子的识别和性质有待进一步研究。阐明滩涂对保护被大肠杆菌等外部细菌污染的自然环境的作用应该是非常有趣的。我们的实验室正在进行这方面的研究。