Metabolic engineering of Escherichia coli for carbon capture and hydrogen storage.

用于碳捕获和氢储存的大肠杆菌代谢工程。

• 批准号: 2869802
• 金额: --
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2869802
• 关键词: Metabolic; engineering; Escherichia; coli; carbon

Empowering a sustainable future through Synthetic Biology: Unleash engineered bacteria's potential for carbon dioxide capture and unlock a sustainable future through hydrogen storage chemical pathways. Rapidly increasing global carbon dioxide levels cause an imminent threat, demanding urgent action for a sustainable future and environmental resilience. This project is focused on engineering a new chassis strain of Escherichia coli that will grow on sources of captured carbon dioxide, including formic acid and methanol, as sole carbon sources. This new chassis will then be further engineered to produce commodity chemicals with an industrial value. Converting waste CO2 into specialist biochemicals using renewable H2 will be a major step towards a circular bioeconomy and achieving Net Zero targets. The project will take three parallel approaches: (1) Engineering of a hydrogen-dependent CO2 reductase where the CO2 and H2 will be converted to formate.Objectives:1. Optimise hydrogen-dependent CO2 reductase activity.2. Enhance captured carbon (formic acid) utilisation in synthetic metabolic pathways.(2) Characterisation of synthetic methylotrophy where the methanol will be converted to formate.Objectives:1. Demonstrate methanol-dependent formate production using engineered enzymes.2. Integrate this pathway with the synthetic reductive glycine pathway.(3) Design, build and test of a novel anaerobic, H2-dependent reductive glycine pathway to facilitate E. coli growth on H2 and CO2 alone, or alternatively methanol or formic acid. Objectives:1. Capturing carbon.2. Utilisation of fixed carbon for bacterial growth.3. Utilisation of fixed carbon for valuable speciality chemicals.Seizing the opportunity, this project introduces pathways for CO2 capture and valuable chemical production, unlocking a sustainable future through engineered bacteria and cutting-edge hydrogen storage pathways.

通过合成生物学实现可持续发展的未来：释放工程菌捕获二氧化碳的潜力，并通过储氢化学途径开启可持续发展的未来。全球二氧化碳水平的迅速上升造成了迫在眉睫的威胁，需要采取紧急行动，以实现可持续的未来和环境复原力。该项目的重点是设计一种新的大肠杆菌底盘菌株，该菌株将在捕获的二氧化碳来源上生长，包括甲酸和甲醇，作为唯一的碳源。这种新的底盘将进一步设计，以生产具有工业价值的商品化学品。利用可再生氢气将废弃的二氧化碳转化为专业的生物化学品将是迈向循环生物经济和实现净零目标的重要一步。该项目将采取三个平行的方法：（1）工程化的氢依赖的CO2还原酶，其中CO2和H2将转化为甲酸盐。目标：1.优化氢依赖性CO2还原酶活性。2.提高合成代谢途径中捕获的碳（甲酸）利用率。(2)甲醇转化为甲酸的合成甲基营养的表征。目的：1.使用工程酶演示甲醇依赖的甲酸盐生产。将该途径与合成还原甘氨酸途径整合。(3)设计、构建和测试一种新的厌氧、H2依赖性还原甘氨酸途径，以促进E.大肠杆菌在单独的H2和CO2上生长，或者在甲醇或甲酸上生长。目的：1.捕获碳。2.利用固定碳进行细菌生长.利用固定碳生产有价值的特种化学品。该项目抓住机遇，引入了二氧化碳捕获和有价值的化学品生产途径，通过工程细菌和尖端储氢途径开启了可持续发展的未来。