Development and Application of Algal Toxicity Test based on Oxygen Evolution

基于析氧的藻类毒性测试方法的开发与应用

• 批准号: 10680511
• 负责人: KANEKO Hidehiro
• 金额: $ 1.34万
• 依托单位: Yamanashi Univerity
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680511/
• 关键词: Algae; Toxicity test; Oxygen evolution; Ecotoxicity

This research aimed to establish an algal toxicity test method based on the algal oxygen evolution rate and to confirm its applicability on to the natural water. Followings are clarified as the result of this research.1. Test method to evaluate algal toxicity in 6 hours was established with the combination of standard test alga, S.capricornutum and one of the standard test culture media AAP medium. This must be useful to evaluate algal toxicity more rapid than the popular one based on algal growth.2. It was clarified that same method could be applied to the most of natural water with native algal species. Because all the algae have the ability to evolve oxygen as a result of photosynthesis. By applying it to natural water, site-specific effects of toxicants that could not be exactly evaluated by standard methods would be examined.3. Actually, some combination of native algae and natural water gave more sensitive results than the combination of S.capricornutum and AAP medium.

本研究旨在建立一种基于藻类放氧速率的藻类毒性测试方法，并验证其在天然水体中的适用性。本研究的主要结果如下：1.采用标准试验藻、羊角藻和标准试验培养基AAP培养基组合，建立了6 h内评价藻类毒性的试验方法。这对于快速评估藻类毒性比基于藻类生长的流行方法更有用。据澄清，相同的方法可以应用于大多数具有本地藻类的天然水。因为所有的藻类都有能力通过光合作用释放氧气。通过将其应用于天然水，可以检查标准方法无法准确评估的有毒物质的特定场地效应。实际上，一些天然藻类和天然水的组合比S.capricornutum和AAP培养基的组合给出更敏感的结果。

项目成果

金子栄廣,竹内英樹,平山公明: "酸素発生速度に基づく迅速な藻類毒性試験"環境毒性学会誌. Vol.13,No.1. 3-9 (2000)

Hidehiro Kaneko、Hideki Takeuchi、Kimiaki Hirayyama：“基于氧气生成率的快速藻类毒性测试”日本环境毒理学会杂志第 13 卷，第 1 期（2000 年）。

竹内英樹,金子栄廣: "酸素発生抑制試験の感度に対するリン・窒素濃度の影響"土木学会第53回年次学術講演会講演概要集. VII. 14-15 (1998)

Hideki Takeuchi、Hidehiro Kaneko：“磷和氮浓度对氧气生成抑制试验灵敏度的影响”日本土木工程学会第 53 届学术年会摘要 VII。

H.TAKEUCH and H.KANEKO: "Effect of phosphorus and nitrogen concentration on the sensitivity of algal oxygen evolution restriction test."Proc.53rd annual meeting of JSCE. 14-15 (1998)

H.TAKEUCH 和 H.KANEKO：“磷和氮浓度对藻类析氧限制测试灵敏度的影响”。JSCE 第 53 届年会。

金子栄廣,財津慎太郎: "藻類酸素発生抑制試験の実環境への適用の試み"第6回バイオアッセイ研究会・日本環境毒性学会合同研究発表会講演要旨集. 27-28 (2000)

Eihiro Kaneko、Shintaro Zaitsu：“尝试将藻类产氧抑制试验应用于真实环境”第 6 届生物测定研究组/日本环境毒理学会联合研究报告摘要 27-28 (2000)。

KANEKO,H.and S.ZAITSU: "Application of algal oxygen evolution restriction test on natural water."Proc.6th annual meeting of JSET. 27-28 (2000)

KANEKO,H. 和 S.ZAITSU：“藻类析氧限制测试在天然水中的应用”。JSET 第六届年会。