Protection Systems in Plant Cells -- A Mechanism of Apoptosis with Monoterpenoids

植物细胞的保护系统——单萜类细胞凋亡机制

• 批准号: 10680568
• 负责人: IZUMI Shunsuke
• 金额: $ 2.3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680568/
• 关键词: Plant; Apoptosis; Monoterpenoids; Signal transduction; Active Oxygen; Chemical Stress; Eliciter; シゲナル伝達; ユリシター

To investigate the defense mechanism of plant cells against chemical stress, we have screened various isoprenoids for inducers of apoptosis in cultured plant cells such as tabacco, chamomile, celery, soybean, and liverwort. These studies have revealed that geraniol has the most potent apoptosis-inducing activity, as judged from DNA fragmentation in the cells. However, NィイD16ィエD1-(ΔィイD12ィエD1-isopentenyl)adenosine and kinetin prevented the geraniol-induced DNA fragmentation in the cells.Furthermore, cycloheximide and actinomycin D, inhibitors of protein and RNA syntheses, respectively, did not suppress the apoptosis of the plant cells. This result suggests that the apoptotic process induced by geraniol does not require the synthesis of new proteins.

为了研究植物细胞对化学胁迫的防御机制，我们在烟草、洋甘菊、芹菜、大豆和肝草等培养的植物细胞中筛选了多种诱导细胞凋亡的类异戊二烯。这些研究表明，香叶醇具有最有效的细胞凋亡诱导活性，从细胞中的DNA片段判断。然而,NィイD16ィエD1 -(ΔィイD12ィエD1-isopentenyl)腺苷和激动素阻止geraniol-induced DNA在细胞分裂。此外，环己亚胺和放线菌素D分别作为蛋白质和RNA合成抑制剂，对植物细胞凋亡没有抑制作用。提示香叶醇诱导的细胞凋亡过程不需要合成新的蛋白。

项目成果

T. Hirata: "A 20-KDa Protein with the GTP-Binding and Trypsin Inhibitory Activity from Glycine max"Biosci. Biotechnol. Biochem.. 63. 1816-1818 (1999)

T. Hirata：“来自大豆的具有 GTP 结合和胰蛋白酶抑制活性的 20-KDa 蛋白质”Biosci。

S. Izumi: "Geroniol is a Potent Inducer of Apoptpsis-like Cell Death in the Cultured Shoot Primordia of Matricaria chamomilla"Biochem. Biophys. Res. Commun.. 259. 519-522 (1999)

S. Izumi：“Geroniol 是母菊培养芽原基中细胞凋亡样细胞死亡的有效诱导剂”Biochem。

T. Hirata: "A symmetric hydrolysis of enol acetates with the Cultured Cells of Marchantia polymorpha"J. Molecular Catalysis B; Enzymatic. 5. 143-147 (1998)

T. Hirata：“用地钱培养细胞进行烯醇乙酸酯的对称水解”J。

T. Hirata: "Epoxidation of Styrene with the peroxidase from the cultured Cells of Nicotiana tabaam"Tetrahedron. 54. 15993-16003 (1998)

T. Hirata：“用来自烟草培养细胞的过氧化物酶对苯乙烯进行环氧化”四面体。