Identification and topology analysis of plant odorant receptors

植物气味受体的鉴定和拓扑分析

• 批准号: 18510186
• 负责人: IZUMI Shunsuke
• 金额: $ 2.56万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18510186/
• 关键词: Odorant receptors; Proteomics; Biotransformation; Membrane topology; Chemical modification; Mass spectrometry; 高等植物; モノテルペノイド

Volatile substances and their receptors was involved in plant-plant communications.We have discussed the roles of terpenes, green odor, Me jasmonate, and plant GPCR (G protein-coupled receptors), two-component system, and chemoreceptor channels as candidates for plant odorant receptors.Next, Protein clusters rule the stereoselectivity of pulegone reductase; the pulegone reductase which bound with β-glucosidase and trypsin inhibitor catalyzed (1R,4R)-isomenthone preferentially rather than (1R,4S)-menthone from (R)-pulegone.Furthermore, the topology and the dynamics of peptide within the liposomes were investigated by use of mass spectrometry combined with acetylation. The peptide within theliposomes was acetylated with NHSSAc; the N-terminal of melittin was acetylated. Thus, it was supposed that the peptide within the liposomes adopted an N-terminal topology.

挥发性物质及其受体参与植物间的交流。我们讨论了萜烯、绿色气味、茉莉酸Me、植物GPCR （G蛋白偶联受体）、双组分系统和化学受体通道作为植物气味受体的候选物质的作用。其次，蛋白质团簇控制了普莱酮还原酶的立体选择性；与β-葡萄糖苷酶和胰蛋白酶抑制剂结合的普莱酮还原酶优先催化(1R,4R)-异薄荷酮，而不是(R)-普莱酮的(1R,4S)-薄荷酮。此外，利用质谱联用乙酰化方法研究了脂质体内肽的拓扑结构和动力学。脂质体内的肽被NHSSAc乙酰化；蜂毒蛋白的n端被乙酰化。因此，假设脂质体内的肽采用n端拓扑结构。

项目成果

Nucleotide excision repair and homologous recombination systems commit differentially to the repair of DNA-Protein crosslinks

• DOI: 10.1016/j.molcel.2007.07.029
• 发表时间: 2007-10-12
• 期刊: MOLECULAR CELL
• 影响因子: 16
• 作者: Nakano, Toshiaki;Morishita, Soh;Ide, Hiroshi
• 通讯作者: Ide, Hiroshi

Topological study of membrane proteins by mass spectrometry.

通过质谱法进行膜蛋白的拓扑研究。

• 发表时间: 2007
• 期刊: J. Mass Spec. Soc. Jpn., 55
• 作者: H. Mizuno;S. Izumi;K. Kominami;and T. Hirata
• 通讯作者: and T. Hirata

Mechanism of the Insertion into Bilayer Membrane by Melittin

蜂毒肽插入双层膜的机制

• 发表时间: 2007
• 作者: K. Saikusa;S. Izumi;T. Hirata
• 通讯作者: T. Hirata

Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from E-coli

• DOI: 10.1016/j.febslet.2006.05.049
• 发表时间: 2006-06-26
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Yamamoto, Tatsuya;Izumi, Shunsuke;Gekko, Kunihiko
• 通讯作者: Gekko, Kunihiko

Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signalling

• DOI: 10.1042/bj20061476
• 发表时间: 2007-03-15
• 期刊: BIOCHEMICAL JOURNAL
• 影响因子: 4.1
• 作者: Kurayoshi, Manabu;Yamamoto, Hideki;Kikuchi, Akira
• 通讯作者: Kikuchi, Akira