Identification and topology analysis of plant odorant receptors

挥发性物质及其受体参与植物间的交流。我们讨论了萜烯、绿色气味、茉莉酸Me、植物GPCR （G蛋白偶联受体）、双组分系统和化学受体通道作为植物气味受体的候选物质的作用。其次，蛋白质团簇控制了普莱酮还原酶的立体选择性；与β-葡萄糖苷酶和胰蛋白酶抑制剂结合的普莱酮还原酶优先催化(1R,4R)-异薄荷酮，而不是(R)-普莱酮的(1R,4S)-薄荷酮。此外，利用质谱联用乙酰化方法研究了脂质体内肽的拓扑结构和动力学。脂质体内的肽被NHSSAc乙酰化；蜂毒蛋白的n端被乙酰化。因此，假设脂质体内的肽采用n端拓扑结构。

Volatile substances and their receptors was involved in plant-plant communications.We have discussed the roles of terpenes, green odor, Me jasmonate, and plant GPCR (G protein-coupled receptors), two-component system, and chemoreceptor channels as candidates for plant odorant receptors.Next, Protein clusters rule the stereoselectivity of pulegone reductase; the pulegone reductase which bound with β-glucosidase and trypsin inhibitor catalyzed (1R,4R)-isomenthone preferentially rather than (1R,4S)-menthone from (R)-pulegone.Furthermore, the topology and the dynamics of peptide within the liposomes were investigated by use of mass spectrometry combined with acetylation. The peptide within theliposomes was acetylated with NHSSAc; the N-terminal of melittin was acetylated. Thus, it was supposed that the peptide within the liposomes adopted an N-terminal topology.