Function analysis of VIP36 related to protein sorting in intracellular transport

VIP36在细胞内转运中与蛋白质分选相关的功能分析

• 批准号: 10680592
• 负责人: KUGE Sayuri
• 金额: $ 1.66万
• 依托单位: SASAKI INSTITUTE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680592/
• 关键词: VIP36; MDCK cell; lectin; high-mannose type sugar chain; intracellular transport

The vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin that recognizes high-mannose type glycan containing α1→2 mannose residue. We isolated the overexpressed clones of VIP36 (+VIP) and the mutant VIP36 (Asp→Asn) (mVIP), which did not show binding activities to high-mannose type glycans by in vitro assay, from MDCK cells. In either clone, VIP36 or mVIP36 was overexpressed in the apical membranes about five times more than in the basolateral ones. In the +VIP clone, apical glycoproteins contained high-mannose sugar chains about four to five times more than basolateral glycoproteins corresponding to the relative same ratios as the VIP36 localization. On the other hand, the glycoproteins containing high-mannose type sugar chains in the mVIP clone were transported to the apical domain two times more than to the basolateral domain as wild type, unrelated to the ratios of overexpressed mVIP36. These results indicated that VIP36 was involved in the intracellular transport of glycoproteins containing high-mannose type sugar chains to the final destinations.

36 kDa囊泡整合蛋白(VIP36)是一种细胞内凝集素，识别含有α-1-→-2甘露糖残基的高甘露糖型糖链。我们从→细胞中分离到高表达的VIP36(+VIP)克隆和突变体VIP36(Asp MVIP Asn)(MVIP)(MVIP)，它们在体外实验中不显示与高甘露糖型多糖结合的活性。在这两个克隆中，VIP36或mVIP36在顶膜中的过度表达大约是在基侧膜中的5倍。在+VIP克隆中，顶端糖蛋白含有的高甘露糖链大约是基侧糖蛋白的四到五倍，与VIP36定位的比例相当。另一方面，mVIP克隆中含有高甘露糖型糖链的糖蛋白被转运到顶端结构域的比例是野生型的两倍，而与mVIP36过表达的比例无关。这些结果表明，VIP36参与了含有高甘露糖型糖链的糖蛋白在细胞内的运输。

项目成果

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Hara-Kuge 等人：“VIP36 凝集素活性与细胞内 N-连接糖蛋白运输相关”Glycoconjugate Journal。

Fukushima, K., Hara-Kuge, S., Seko, A., Ikehara, Y., and Yamashita, K.: "Elevation of Gal : α2→6 sialyltransferase and Gal : α1→2 fucosyltransferase activities in human choriocarcinoma"Cancer Res.. 58. 4301-4306 (1998)

Fukushima, K.、Hara-Kuge, S.、Seko, A.、Ikehara, Y. 和 Yamashita, K.：“人类绒毛膜癌中 Gal：α2→6 唾液酸转移酶和 Gal：α1→2 岩藻糖基转移酶活性的升高”癌症决议.. 58. 4301-4306 (1998)

Fukushima, K., Hara-Kuge, S. and Yamashita, K.: "Functional roles of high-mannose type glycans on IL-2-dependent CTLL-2 cell proliferation"(Submitted for publication).

Fukushima, K.、Hara-Kuge, S. 和 Yamashita, K.：“高甘露糖型聚糖对 IL-2 依赖性 CTLL-2 细胞增殖的功能作用”（已提交出版）。

Yamashita, K . et al.: "Intracellular lectins associated with N-linked giycoprotein traffic"Biochem. Biochim. Acta. 1473. 147-160 (1999)

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Seko, A.、Hara-Kuge, S.、Yonezawa, S.、Nagata, K. 和 Yamashita, K.：“N-乙酰葡糖胺-6-O-硫酸盐特异性 β1,4-半乳糖基转移酶的鉴定和表征人结直肠粘膜”FEBS Lett.. 440. 307-310 (1998)