Genetic operation of mouse embryos using exo-utero surgery

利用宫外手术对小鼠胚胎进行基因操作

• 批准号: 10680752
• 负责人: KAWANO Hitoshi
• 金额: $ 2.56万
• 依托单位: Tokyo Metropolitan Organization for Medical Research (1999)Tokyo Metropolitan Institute for Neuroscience (1998)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680752/
• 关键词: exo-utero surgery; mouse; development; antisense DNA; L1; TAG-1; proteoglycan; brain

Recent development in the field of genetic and molecular technology has remarkably increased the knowledge on the mechanisms of the brain development. However, experimental approach to mammalian brain has been with much difficulty, because mammalian embryos develop in mother's uterus. To overcome this problem, we have developed the technique of the intrauteral surgery of mouse embryos.Mice of 11 and 12 days of pregnancy was laparotomised and the adenovirus vector bearing Lac Z were demonstrated using x-gal staining. X-gal-positive cells were localized in the choroid plexus and a migrating rout from the rhombic lip to the cerebellar surface. These results indicate that the intrauteral surgery combined with adenovirus vector is useful tool to investigate the mechanisms underlying mammalian brain development.

遗传和分子技术领域的最新发展已大大提高了对大脑发育机制的知识。然而，哺乳动物大脑的实验方法很困难，因为哺乳动物的胚胎在母亲的子宫中发展。为了克服这个问题，我们已经开发了小鼠胚胎内手术的技术。将怀孕11天和12天的小鼠拉帕罗特术，并使用X-gal染色证明了腺病毒载体载有lac z的腺病毒载体。 X-Gal阳性细胞位于脉络丛中，并从菱形唇向小脑表面迁移。这些结果表明，与腺病毒载体相结合的田间手术是研究哺乳动物脑发育的基础机制的有用工具。

项目成果

川野 仁、他: "共立出版"脳と神経・分子生物学入門. 8 (1999)

Hitoshi Kawano 等人：“Kyoritsu Shuppan”脑与神经学/分子生物学简介 8 (1999)。

Kawamura K. et al.: "Keio University Symposium for Life Science and Medicine, Vol. 2. Neural Development"Springer-Verlag, Tokyo. 10 (1999)

Kawamura K. 等人：“庆应义塾大学生命科学与医学研讨会，第 2 卷。神经发育”Springer-Verlag，东京。

Yamamoto A et al.: "Deficiency in protein L-isoaspartyl methyltransferase results in a fetal progressive epilepcy." J.Neurosci.18. 2063-2074 (1998)

Yamamoto A 等人：“蛋白质 L-异天冬氨酰甲基转移酶缺乏会导致胎儿进行性癫痫。”

川野 仁,他: "ラット大脳皮質における神経路形成の分子メカニズム" 日本神経精神薬理. (印刷中). (1999)

Hitoshi Kawano 等人：“大鼠大脑皮层神经通路形成的分子机制”，日本神经精神药理学（出版中）。

Yamamoto A et al.: "Deficiency in protein L-isoaspartyl methyltransferase results in a fetal progressive epilepcy."J. Neurosci.. 18. 2063-2074 (1998)

Yamamoto A 等人：“蛋白质 L-异天冬氨酰甲基转移酶缺乏会导致胎儿进行性癫痫。”J.