Genetic operation of mouse embryos using exo-utero surgery

利用宫外手术对小鼠胚胎进行基因操作

• 批准号: 10680752
• 负责人: KAWANO Hitoshi
• 金额: $ 2.56万
• 依托单位: Tokyo Metropolitan Organization for Medical Research (1999)Tokyo Metropolitan Institute for Neuroscience (1998)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680752/
• 关键词: exo-utero surgery; mouse; development; antisense DNA; L1; TAG-1; proteoglycan; brain

Recent development in the field of genetic and molecular technology has remarkably increased the knowledge on the mechanisms of the brain development. However, experimental approach to mammalian brain has been with much difficulty, because mammalian embryos develop in mother's uterus. To overcome this problem, we have developed the technique of the intrauteral surgery of mouse embryos.Mice of 11 and 12 days of pregnancy was laparotomised and the adenovirus vector bearing Lac Z were demonstrated using x-gal staining. X-gal-positive cells were localized in the choroid plexus and a migrating rout from the rhombic lip to the cerebellar surface. These results indicate that the intrauteral surgery combined with adenovirus vector is useful tool to investigate the mechanisms underlying mammalian brain development.

近年来遗传和分子技术领域的发展显著增加了对大脑发育机制的认识。然而，由于哺乳动物胚胎是在母体子宫内发育的，因此对哺乳动物大脑的实验研究一直存在很大的困难。为了克服这一问题，我们开发了小鼠胚胎腔内手术技术。对妊娠11天和12天的小鼠进行剖腹探查，用x-gal染色法检测携带Lac Z的腺病毒载体。阳性细胞定位于脉络膜丛，并从菱形唇向小脑表面迁移。这些结果表明，腔内手术结合腺病毒载体是研究哺乳动物脑发育机制的有用工具。

项目成果

川野 仁、他: "共立出版"脳と神経・分子生物学入門. 8 (1999)

Hitoshi Kawano 等人：“Kyoritsu Shuppan”脑与神经学/分子生物学简介 8 (1999)。

川野 仁,他: "ラット大脳皮質における神経路形成の分子メカニズム" 日本神経精神薬理. (印刷中). (1999)

Hitoshi Kawano 等人：“大鼠大脑皮层神经通路形成的分子机制”，日本神经精神药理学（出版中）。

Kawamura K. et al.: "Keio University Symposium for Life Science and Medicine, Vol. 2. Neural Development"Springer-Verlag, Tokyo. 10 (1999)

Kawamura K. 等人：“庆应义塾大学生命科学与医学研讨会，第 2 卷。神经发育”Springer-Verlag，东京。

Yamamoto A et al.: "Deficiency in protein L-isoaspartyl methyltransferase results in a fetal progressive epilepcy." J.Neurosci.18. 2063-2074 (1998)

Yamamoto A 等人：“蛋白质 L-异天冬氨酰甲基转移酶缺乏会导致胎儿进行性癫痫。”

Kawano H et al.: "Renerating axons of the paraventriculo-neurohypophysial tract invaded the scar tissue that expresses extracellular matrix molecules"J. Brain Sci.. 23. 241-249 (1997)

Kawano H 等人：“室旁神经垂体束的再生轴突侵入了表达细胞外基质分子的疤痕组织”J.