Genetic operation of mouse embryos using exo-utero surgery
Genetic operation of mouse embryos using exo-utero surgery
批准号:
10680752
负责人:
KAWANO Hitoshi
金额:
$2.56万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1998
资助国家:
日本
项目状态:
已结题
起止时间:
1998 至 1999
中文摘要
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英文摘要
Recent development in the field of genetic and molecular technology has remarkably increased the knowledge on the mechanisms of the brain development. However, experimental approach to mammalian brain has been with much difficulty, because mammalian embryos develop in mother's uterus. To overcome this problem, we have developed the technique of the intrauteral surgery of mouse embryos.Mice of 11 and 12 days of pregnancy was laparotomised and the adenovirus vector bearing Lac Z were demonstrated using x-gal staining. X-gal-positive cells were localized in the choroid plexus and a migrating rout from the rhombic lip to the cerebellar surface. These results indicate that the intrauteral surgery combined with adenovirus vector is useful tool to investigate the mechanisms underlying mammalian brain development.
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川野 仁、他: "共立出版"脳と神経・分子生物学入門. 8 (1999)
Hitoshi Kawano 等人:“Kyoritsu Shuppan”脑与神经学/分子生物学简介 8 (1999)。
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通讯作者:
川野 仁,他: "ラット大脳皮質における神経路形成の分子メカニズム" 日本神経精神薬理. (印刷中). (1999)
Hitoshi Kawano 等人:“大鼠大脑皮层神经通路形成的分子机制”,日本神经精神药理学(出版中)。
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Kawamura K. et al.: "Keio University Symposium for Life Science and Medicine, Vol. 2. Neural Development"Springer-Verlag, Tokyo. 10 (1999)
Kawamura K. 等人:“庆应义塾大学生命科学与医学研讨会,第 2 卷。神经发育”Springer-Verlag,东京。
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Yamamoto A et al.: "Deficiency in protein L-isoaspartyl methyltransferase results in a fetal progressive epilepcy." J.Neurosci.18. 2063-2074 (1998)
Yamamoto A 等人:“蛋白质 L-异天冬氨酰甲基转移酶缺乏会导致胎儿进行性癫痫。”
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Kawano H et al.: "Renerating axons of the paraventriculo-neurohypophysial tract invaded the scar tissue that expresses extracellular matrix molecules"J. Brain Sci.. 23. 241-249 (1997)
Kawano H 等人:“室旁神经垂体束的再生轴突侵入了表达细胞外基质分子的疤痕组织”J.
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