Morphological analyses about origins, neurochemical substances and target-specificily of neurons coming to the subfornical organ

用顺行和逆行追踪技术结合免疫组织化学方法研究穹隆下器(SFO)神经元的来源、神经化学物质和靶向性。为研究的基础，对含有多种神经化学物质的神经末梢的分布和靶成分进行了研究。SFO内有氨基能、胆碱能和肽能神经末梢。5-羟色胺、甲硫氨酸脑啡肽-8(ME8)、甘丙素(GAL)、神经降压素和黄体激素释放激素免疫反应阳性神经末梢与胞体和树突形成突触，并与SFO内的血管和神经胶质细胞密切接触。ME8免疫反应阳性投射起源于前背侧和前腹侧室周核、视前核和下丘脑室周区、正中视前核、下丘脑背内侧和弓状核、臂旁内侧核和孤束核，GAL免疫反应阳性投射来自视前内侧核、室旁内侧核、下丘脑室周区、下丘脑背内侧和弓状核，冰片样免疫反应阳性投射来自下丘脑室旁内侧核和延髓头端腹外侧核，神经肽Y免疫反应阳性投射来自下丘脑弓状核、孤立核和延髓腹外侧区，胆碱能投射来自内侧隔核和斜角带。从正中视前核到SFO的投射很大。它们与胞体和树突形成突触，并与血管和胶质细胞密切接触。明确SFO神经元投射到主要靶点之一的室旁核与主要来源的神经末梢(如正中视前核、臂旁外侧核和中缝核)之间的关系是非常有趣的。这是要解决的问题。

Origins, neurochemical substances and target-specificity of neurons coming to the subfornical organ(SFO) were investigated using anterograde and retrograde tracing technique combined with immunohistochemistry. Distribution and target elements of nerve terminals containing several neurochemical substances were investigated for the basis of the study. There were aminergic, cholinergic and peptidergic nerve terminals in the SFO. Serotonin-,Met-enkephalin-8(mE8)-,galanin(GAL)-,neurotensin- and luteinizing hormone-releasing hormone-immunoreactive nerve terminals made synapses with cell bodies and dendrites, and contacted closely with vessels and glial cells in the SFO. Origins of mE8-immunoreactive projections were the anterodorsal and anteroventral periventricular nuclei, preoptic and hypothalamic periventricular regions, median preoptic nucleus, hypothalamic dorsomedial and arcuate nuclei, medial parabrachial nucleus and solitary nucleus ; GAL-immunoreactive projections were the medial preoptic nucleus, medial parvocellular paraventricular nucleus, hypothalamic periventricular regions, hypothalamic dorsomedial and arcuate nuclei ; bornbesin-immunoreactive projections were the medial parvocellular paraventricular nucleus and rostral ventrolateral medulla ; neuropeptide Y-immunoreactive projections were the hypothalamic arcuate nucleus, solitary nucleus and ventrolateral medulla ; cholinergic projections were the medial septal nucleus and diagonal band. Projections to the SFO from the median preoptic nucleus were huge. They made synapses with cell bodies and dendrites, and contacted closely with vessels and glial cells.It is very interesting to clear the relationship between SFO neurons projecting to one of the main targets, the paraventricular nucleus and nerve terminals coming from main origins, such as the median preoptic nucleus, lateral parabrachial nucleus and raphe nuclei. This is the matter to be solved.