Morphological analyses about origins, neurochemical substances and target-specificily of neurons coming to the subfornical organ

关于穹窿下器官神经元的起源、神经化学物质和目标特异性的形态学分析

• 批准号: 14580771
• 负责人: KAWANO Hitoshi
• 金额: $ 1.92万
• 依托单位: Saga University (2004)佐賀医科大学 (2002-2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580771/
• 关键词: subfornical organ; nerve inputs; origins; neurochemical substances; axonal transport technique; immunohistochemistry; electron microscopy; 標的要素

Origins, neurochemical substances and target-specificity of neurons coming to the subfornical organ(SFO) were investigated using anterograde and retrograde tracing technique combined with immunohistochemistry. Distribution and target elements of nerve terminals containing several neurochemical substances were investigated for the basis of the study. There were aminergic, cholinergic and peptidergic nerve terminals in the SFO. Serotonin-,Met-enkephalin-8(mE8)-,galanin(GAL)-,neurotensin- and luteinizing hormone-releasing hormone-immunoreactive nerve terminals made synapses with cell bodies and dendrites, and contacted closely with vessels and glial cells in the SFO. Origins of mE8-immunoreactive projections were the anterodorsal and anteroventral periventricular nuclei, preoptic and hypothalamic periventricular regions, median preoptic nucleus, hypothalamic dorsomedial and arcuate nuclei, medial parabrachial nucleus and solitary nucleus ; GAL-immunoreactive projections were the medial preoptic nucleus, medial parvocellular paraventricular nucleus, hypothalamic periventricular regions, hypothalamic dorsomedial and arcuate nuclei ; bornbesin-immunoreactive projections were the medial parvocellular paraventricular nucleus and rostral ventrolateral medulla ; neuropeptide Y-immunoreactive projections were the hypothalamic arcuate nucleus, solitary nucleus and ventrolateral medulla ; cholinergic projections were the medial septal nucleus and diagonal band. Projections to the SFO from the median preoptic nucleus were huge. They made synapses with cell bodies and dendrites, and contacted closely with vessels and glial cells.It is very interesting to clear the relationship between SFO neurons projecting to one of the main targets, the paraventricular nucleus and nerve terminals coming from main origins, such as the median preoptic nucleus, lateral parabrachial nucleus and raphe nuclei. This is the matter to be solved.

采用正反行示踪技术结合免疫组织化学技术，研究了神经细胞的来源、神经化学物质和靶向特异性。研究了含多种神经化学物质的神经末梢的分布和靶元素，为研究奠定了基础。SFO有胺能、胆碱能和肽能神经末梢。5 -羟色胺-、Met-enkephalin-8(mE8)-、甘丙氨酸(GAL)-、神经紧张素-和黄体生成素释放激素免疫反应性神经末梢与细胞体和树突形成突触，并与SFO的血管和胶质细胞紧密接触。me8免疫反应性投射的来源为前鼻侧和前腹侧脑室周围核、视前区和下丘脑脑室周围区、正中视前核、下丘脑背内侧核和弓形核、内侧臂旁核和孤立核；gal免疫反应性投影为内侧视前核、内侧室旁旁细胞核、下丘脑室周区、下丘脑背内侧核和弓形核；bornbesin免疫反应性突起为内侧室旁旁细胞核和延髓吻侧腹外侧；神经肽y免疫反应性突起为下丘脑弓状核、孤立核和腹外髓质；胆碱能突起为内侧隔核和斜带。视前正中核向SFO的突起很大。它们与细胞体和树突形成突触，并与血管和神经胶质细胞紧密接触。明确投射到主要靶点之一室旁核的SFO神经元与来自主要来源的神经末梢（如正中视前核、外侧臂旁核和中缝核）之间的关系是非常有趣的。这就是要解决的问题。