Hox genes of the direct-type developing sea urchin Peronella japonica

直接型发育海胆Peronella japonica的Hox基因

• 批准号: 10836009
• 负责人: YAMAGUCHI Masaki
• 金额: $ 2.18万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10836009/
• 关键词: Hox gene; echinoderm; direct-type developer; sea urchin; Peronella japonica; Evx-type gene; 直接発生型ワニ; HOX遺伝子; 進化; Para HOX遺伝子; 五放射相称; 前後軸

Peronella japonica is a direct-type developing sea urchin that metamorphoses in 3 days without feeding, providing us a good system to analyze developmental mechanisms especially at later larval stages to metamorphosis. We surveyed the Hox-type sequences of P. japonica by PCR and RT-PCR, and isolated 13 Hox-type sequences. All of them were detected by RT-PCR, indicative of their expressions in larvae. Phylogenetic analysis and database searches resulted in identification of all the P. japonica orthologs of the Hox genes that have been reported in other sea urchins, plus 3 Hox-type genes: the Evx-type, Xlox-type, and an additional posterior Hox gene. The present results revealed a putative Hox gene cluster of P. japonica, which contains genes representing all the paralog (cognate) groups of vertebrate Hox clusters except that there is a single gene of Hox4-5 types. In spite of the pentaradial body plans lacking apparent cepharic structures, P. japonica appears to retain the Hox cluster that is essentially similar to those of the chordates with bilateral body plans.

Peronella japonica 是一种直接型发育的海胆，在不喂食的情况下在 3 天内变态，为我们提供了一个良好的系统来分析发育机制，特别是在幼虫后期到变态阶段。我们通过PCR和RT-PCR技术对粳稻Hox型序列进行了调查，分离得到13条Hox型序列。所有这些都通过 RT-PCR 检测到，表明它们在幼虫中的表达。系统发育分析和数据库搜索结果鉴定出在其他海胆中已报道的 Hox 基因的所有日本 P. japonica 直系同源物，以及 3 个 Hox 型基因：Evx 型、Xlox 型和一个额外的后 Hox 基因。目前的结果揭示了日本P. japonica的假定Hox基因簇，其包含代表脊椎动物Hox簇的所有旁系同源（同源）群体的基因，除了Hox4-5类型的单个基因之外。尽管五桡体的身体计划缺乏明显的头颅结构，日本对虾似乎保留了与双侧身体计划的脊索动物基本相似的 Hox 簇。

项目成果

Yamaguchi, S. et al.: "The Hox genes of the direct-type developing seam urchin Peronella japonica"Zygote. (in press). (2000)

Yamaguchi, S. 等人：“直接型发育的缝海胆 Peronella japonica 的 Hox 基因”合子。

Yamaguchi, S otal: "The Hox genes of the direct-type developing sea urchin Peronella japonica"Zygote. in press. (2000)

Yamaguchi, S otal：“直接型发育海胆 Peronella japonica 的 Hox 基因”合子。

Yamaguchi, S. et al.: "The Hox Gene of the Direct-type Developing Sea Unhin Peranella japonica"Zygote. (in press). (2000)

Yamaguchi, S.等人：“直接型发育海Unhin Peranella japonica”合子的Hox基因。