Immunosensor with Fluid Control Mechanism for Salivary Cortisol Analysis

用于唾液皮质醇分析的具有流体控制机制的免疫传感器

• 批准号: 22500400
• 负责人: YAMAGUCHI Masaki
• 金额: $ 2.75万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500400/
• 关键词: 計測工学; 生物・生体工学; 分子認識; 流体工学; ストレス

The clinical utility of salivary cortisol measurements has been restricted by the lack of appropriate technology platforms that allow near real-time" detection and quantification of salivary cortisol. Integrating early identification and risk stratification in patients with stress-related diseases will require that care providers have timely access to salivary stress biomarker data. The purpose of this research is to demonstrate a new design for a cortisol immunosensor for the noninvasive and quantitative analysis of salivary cortisol. We propose a cortisol immunosensor with a fluid control mechanism which has both a vertical flow and a lateral flow. The detected current resulting from a competitive reaction between the sample cortisol and a glucose oxidase-labeled cortisol conjugate was found to be inversely related to the concentration of cortisol in the sample solution. The measurement could be accomplished within 35 minutes and the cortisol immunosensor could be reused. Additionally, a novel saliva collection kit that can fix the saliva volume with only a four-step action was proposed. Used for evaluation of human salivary cortisol levels, the cortisol immunosensor measurement corresponded closely with commercially available ELISA method R^2 = 0.92) for a range of the concentrations of native salivary cortisol (0.1 . 10 ng/ml). Our results indicate the promise of the new cortisol immunosensor for noninvasive, point-of care measurement of human salivary cortisol levels.

唾液皮质醇测量的临床应用受到了限制，因为缺乏适当的技术平台，可以近实时检测和量化唾液皮质醇。整合与压力相关疾病患者的早期识别和风险分层将需要护理提供者及时获得唾液应激生物标志物数据。本研究的目的是展示一种新的设计皮质醇免疫传感器的非侵入性和定量分析唾液皮质醇。我们提出了一种皮质醇免疫传感器与流体控制机制，它具有垂直流动和横向流动。发现由样品皮质醇和葡萄糖氧化酶标记的皮质醇缀合物之间的竞争反应产生的检测电流与样品溶液中皮质醇的浓度成反比。该方法可在35 min内完成测量，且传感器可重复使用。此外，还提出了一种新型的唾液收集试剂盒，该试剂盒可以仅通过四步动作来固定唾液体积。用于评估人唾液皮质醇水平，皮质醇免疫传感器测量结果与市售ELISA方法（R^2 = 0.92）在天然唾液皮质醇浓度范围（0.1 . 10 ng/ml）。我们的研究结果表明，新的皮质醇免疫传感器的承诺，无创，点的护理测量人类唾液皮质醇水平。

项目成果

体外診断用医薬品の開発と承認申請,第10章12節 唾液バイオマーカーによる非侵襲検査のニーズとシーズ

体外诊断药物的开发和申请批准，第十章第十二节利用唾液生物标志物进行无创检测的需求和种子

• 发表时间: 2010
• 作者: Ayano E;Karaki M;Ishihara T;KanazawaH;Okano T. Poly;菅原 隆編,山口 昌樹(分担執筆)
• 通讯作者: 菅原 隆編,山口 昌樹(分担執筆)

Oral and Maxillofacial Surgery Clinics of North America, Collaborative Care of the Facial Injury Patient, - Salivary Biosensors for Screening Trauma related Psychopathology-

北美口腔颌面外科诊所，面部损伤患者的协作护理，-用于筛查创伤相关精神病理学的唾液生物传感器-

• 发表时间: 2010
• 作者: Vivek Shetty and Grant N. Marshall Eds.;Vivek Shetty and Masaki Yamaguchi
• 通讯作者: Vivek Shetty and Masaki Yamaguchi

救急医療従事者の夜間勤務が唾液アミラーゼ活性の日内変動へもたらす影響

急诊医务人员夜班对唾液淀粉酶活性日波动的影响

• 发表时间: 2011
• 期刊: 日本救急医学会雑誌
• 作者: 水野理介;一色政志;藤田敏郎;Eiki Takahashi;中島 康,高橋 貴之,山口 昌樹
• 通讯作者: 中島 康,高橋 貴之,山口 昌樹

流体制御機構を備えたコルチゾールセンサの検出電流の評価

具有流体控制机构的皮质醇传感器检测电流评估

• 发表时间: 2011
• 作者: Dhermendar;K. Tiawari;Takashi Jin;and J Behari;佐々木 翔平,松田 陽平,今井 義勝,丹羽 大介,佐々木 誠,山口 昌樹
• 通讯作者: 佐々木 翔平,松田 陽平,今井 義勝,丹羽 大介,佐々木 誠,山口 昌樹

Patterns of salivary cortisol levels can manifest work stress in emergency care providers.

• DOI: 10.1007/s12576-012-0197-8
• 发表时间: 2012-05
• 期刊: JOURNAL OF PHYSIOLOGICAL SCIENCES
• 影响因子: 2.3
• 作者: Nakajima, Yasushi;Takahashi, Takayuki;Shetty, Vivek;Yamaguchi, Masaki
• 通讯作者: Yamaguchi, Masaki