Effect of electromagnetic field on sperm movement (Orientation of sperm in static magnetic field)

用显微观察方法研究了公牛精子在静磁场中的取向。公牛精子有一个非常扁平的头部，它们是固定的，它们的运动被戊二醛阻止。它们的方向是整个身体和垂直于磁场方向的平头。抗磁性细胞成分，如细胞膜、头部的DNA和尾部的微管，被认为对这种定向有贡献，因为公牛精子没有顺磁性成分。为了定量测量取向，测定了在光度池中通过戊二醛固定的牛精子悬浮液的透射光强度。强度的变化与精子的平均定向程度成比例。它随着磁场强度的增加而显著增加，在大约1t时达到100%。与红细胞或血小板相比，磁性非常强。通过研究公牛精子对磁场的反应，将有可能更详细地了解其微观结构。

The orientation of bull sperms in static magnetic fields was investigated by microscopic observation. The bull sperms, which have a very flat head, were fixed and their motion was stopped by glutaraldehyde. They were oriented with the whole body and the flat head perpendicular to the direction of the magnetic field. The diamagnetic cell components, such as the cell membrane, the DNA in the head, and the microtubule in the tail, were thought to contribute to this orientation, because bull sperms do not have paramagnetic components.For quantitative measurement of the orientation, the intensity of transmitted light through glutaraldehyde-fixed bull sperm suspension in a photometric cell was determined. The intensity changes slightly in proportion to the mean degree of orientation of the sperms. It increased sigmoidally depending on the intensity of the magnetic field and reached 100% at almost 1 T.The magnetism is very strong in comparison to that of erythrocytes or platelets.By studying the response of the bull sperm to the magnetic field, it will be possible to understand its microstructure in more detail.