Effect of electromagnetic field on sperm movement (Orientation of sperm in static magnetic field)

电磁场对精子运动的影响（静态磁场中精子的方向）

• 批准号: 10837008
• 负责人: HIGASHI Terumasa
• 金额: $ 2.11万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10837008/
• 关键词: sperm; static magnetic field; magnetic orientation; diamagnetism; anisotropic magnetic susceptivility; flagellum; paramecium; cilium; 電磁気学; 生体磁気学; 磁場影響

The orientation of bull sperms in static magnetic fields was investigated by microscopic observation. The bull sperms, which have a very flat head, were fixed and their motion was stopped by glutaraldehyde. They were oriented with the whole body and the flat head perpendicular to the direction of the magnetic field. The diamagnetic cell components, such as the cell membrane, the DNA in the head, and the microtubule in the tail, were thought to contribute to this orientation, because bull sperms do not have paramagnetic components.For quantitative measurement of the orientation, the intensity of transmitted light through glutaraldehyde-fixed bull sperm suspension in a photometric cell was determined. The intensity changes slightly in proportion to the mean degree of orientation of the sperms. It increased sigmoidally depending on the intensity of the magnetic field and reached 100% at almost 1 T.The magnetism is very strong in comparison to that of erythrocytes or platelets.By studying the response of the bull sperm to the magnetic field, it will be possible to understand its microstructure in more detail.

用显微镜观察了公牛精子在静磁场中的取向。将头部非常扁平的公牛精子固定，并用戊二醛阻止其运动。它们的整个身体和扁平头部垂直于磁场的方向。由于牛精子不具有顺磁性成分，因此认为细胞膜、头部的DNA和尾部的微管等抗磁性成分对牛精子的取向有一定的影响。这种强度的变化与精子的平均取向度成正比。根据磁场强度的不同，精子的磁性呈S形增加，在1 T时几乎达到100%。与红细胞或血小板相比，其磁性非常强。通过研究公牛精子对磁场的反应，可以更详细地了解其微观结构。

项目成果

R.EMURA (et. al.): "Orientation of bull sperms in static magnetic field."Bioelectromagnetics. 22(1). 60-65 (2001)

R.EMURA（等人）：“静态磁场中公牛精子的方向。”生物电磁学。

T.HIGASHI (et.al): "Effects of magnetic field on blood Elythrocytes."Applied Physics. 67(10). 1142-1145 (1998)

T.HIGASHI（等人）：“磁场对血红细胞的影响。”应用物理学。

R.EMURA (et.al): "Bull sperm orientation in strong static magnetic fields."J.JPN.Bioelectromagnetic Society. 12(1). 194-195 (1999)

R.EMURA（等人）：“公牛精子在强静磁场中的定向。”J.JPN.生物电磁学会。

東照正(等): "鞭毛・繊毛の磁場配向"Symposium on New Magneto-Science 2000, Proceedings of the Fourth Meeting. 1(1). 196-202 (2000)

Masaaki Azuma (等人)：“鞭毛和纤毛的磁场定向”2000 年新磁科学研讨会，第四次会议记录 1(1) (2000)。

江村玲奈(等): "ウシ精子の定常磁場配向"日本生体磁気学会. 12・1. 194-195 (1999)

Rena Emura (et al.)：“牛精子的稳定磁场定向”日本生物磁学会 12・195 (1999)。