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Peripheral neuronal mechanisms for control of the gill movements in Aplysia

控制海兔鳃运动的周围神经机制

基本信息

批准号：
11168225
负责人：
KUROKAWA Makoto
金额：
$ 12.22万
依托单位：
Tokyo Metropolitan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas (A)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11168225/
关键词：
Aplysia Gill movement L7 Branchial ganglion Abdominal ganglion Peripheral nervous system Serotonin Anti-L7 軟体動物神経集網学習鰓 SCP_B

项目摘要

L7 is a gill motor neuron which drives gill longitudinal shortening in Aplysia californica, A. kurodai, and A. juliana. We have identified a neuron in the right hemisphere of the abdominal ganglion, referred to as Arti-L7, which opposes L7-driven longitudinal shortening in the species. Anti-L7 did not block the generation of L7 impulses within the abdominal ganglion but apparently opposed L7-driven longitudinal shortening at the periphery of the gill. The spontaneous firing rate of Anti-L7 impulses was in a range below about 5 Hz. The suppression of L7-driven longitudinal shortening was dependent on the firing rate of Anti-L7. The threshold firing rate of Anti-L7 impulses for the suppressive effect was less than 0.5 Hz. In response to electrical stimulus pulses applied to the pleuro-abdominal connective, the impulse firing of Anti-L7 was suppressed, while a long-lasting hyperpolarizing potential occurred in the neuron. The suppression continued for several minutes even after the stimulation ceased. During the suppression, the L7-driven longitudinal shortening of the gill and gill movements induced by stimulation of the siphon nerve were potentiated. The results may show that sensitization of the gill withdrawal reflex is, at least in part, attributable to suppression of Anti-L7 activity.Immunocytochemical analysis revealed that Anti-L7 shows serotonin-immunoreactivity. The location, the serotonin immunoreactivity and the electrophysiological properties of Anti-L7 indicate that it is identical to a member of the RB neuron cluster known in A. californica. In the gill, serotonin-immunoreactive neural processes were observed for muscle cells, branchial-ganglionic neurons and plexus neurons. These sites may be involved as loci of mechanisms for antagonistic action of Anti-L7 on L7-induced gill movements.

L7是一个驱动鳃纵向缩短的鳃运动神经元。kurodai和A.朱莉安娜我们已经在腹神经节的右半脑中确定了一个神经元，称为Arti-L7，它反对L7驱动的物种纵向缩短。抗L7并没有阻止产生的L7脉冲内的腹神经节，但显然反对L7驱动的纵向缩短在鳃的周边。抗L7脉冲的自发放电率在低于约5Hz的范围内。对L7驱动的纵向缩短的抑制依赖于抗L7的放电率。抑制效应的抗L7脉冲的阈值放电率小于0.5 Hz。响应于施加到胸腹结缔组织的电刺激脉冲，抗L7的脉冲放电被抑制，而在神经元中发生持久的超极化电位。即使在刺激停止后，抑制仍持续数分钟。在抑制过程中，L7驱动的鳃的纵向缩短和刺激虹吸神经引起的鳃运动增强。结果表明，退鳃反射的敏感化至少部分归因于抗L7活性的抑制。免疫细胞化学分析表明，抗L7显示了胡萝卜素的免疫反应性。抗L7的定位、5-羟色胺免疫反应性和电生理特性表明它与A.加利福尼亚州。在鳃中，观察到肌细胞、鳃神经节神经元和神经丛神经元的降钙素免疫反应阳性神经突起。这些位点可能作为抗L7对L7诱导的鳃运动的拮抗作用的机制的位点。