Biochemical studies on the quantum mechanical successive reaction system in organized molecular assemblies in biological materials.

分析了光合作用初级过程中的连续反应系统。我们使用的是生物材料，而不是构建人工系统。我们得到了以下四个主要结果：(1)分析了一种新发现的含有Chld的蓝藻Acaryo氯is marina的能量传递过程，天线Chla的能级低于能量陷阱，但证明了上坡能量传递过程在该生物体中起作用，这表明天线系统中有一个新的概念。我们还发现了一个含有Chl d的新物种，从而最终得出了在红藻中存在Chl d的结论。(2)我们证明了新发现的含有光合细菌Acidiphilium rubrum的锌-BChl a中的特殊对的空间取向与紫色细菌中的不同。通过对蓝藻天线蛋白，即藻胆蛋白的简正波分析，得出蛋白质结构对于稳定参与后续反应体系的发色团的电子态是必不可少的结论。(4)分析了类胡萝卜素的激发弛豫过程，发现类胡萝卜素作为藻类中的一种有效的天线，在S2态的快速振动弛豫对天线的作用是必不可少的。

The successive reaction system in the primary processes of photosynthesis was analyzed. We used biological materials, and did not construct the artificial system. We obtained the following four major results.(1) We analyzed the energy transfer process in a newly found cyanobacterium Acaryochloris marina that is known to contain Chl d. The energy level of antenna Chl a is lower than the energy trap, however the up-hill energy transfer process was proved to function in this organism, indicating a new concept in the antenna system. We also found a new species that contains Chl d, and this leads to the final conclusion on the presence of Chl d in red algae.(2) We proved that the spatial orientation of the special pair in the newly found Zn-BChl a containing photosynthetic bacterium Acidiphilium rubrum was different from that in the purple bacteria. A weak interaction between Zn-BChl a's suggested the modification in the reaction kinetics.(3) we applied the normal mode analysis to the antenna protein of cyanobacteria, that is, phycobiliproteins, and concluded that the protein structure is essential for stabilizing the electronic state of chromophores that engages in the successive reaction system.(4) We analyzed the excitation relaxation processes of carotenoids that function as an efficient antenna in algae, and found that very fast vibrational relaxationin the S2 state is essential for the antenna function.