Biochemical studies on the quantum mechanical successive reaction system in organized molecular assemblies in biological materials.

生物材料中有序分子组装体中量子力学连续反应系统的生化研究。

• 批准号: 11223206
• 负责人: MIMURO Mamoru
• 金额: $ 26.94万
• 依托单位: YAMAGUCHI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11223206/
• 关键词: Photosynthesis; Energy transfer; successive reaction system; Reaction center; novel pigments; 量子化反応; 超高速分光法; カロテノイド; 緩和過程; 連鎖反応; 量子論; アンテナ; 緑色光合成細菌; クロロゾーム; フェムト秒分光

The successive reaction system in the primary processes of photosynthesis was analyzed. We used biological materials, and did not construct the artificial system. We obtained the following four major results.(1) We analyzed the energy transfer process in a newly found cyanobacterium Acaryochloris marina that is known to contain Chl d. The energy level of antenna Chl a is lower than the energy trap, however the up-hill energy transfer process was proved to function in this organism, indicating a new concept in the antenna system. We also found a new species that contains Chl d, and this leads to the final conclusion on the presence of Chl d in red algae.(2) We proved that the spatial orientation of the special pair in the newly found Zn-BChl a containing photosynthetic bacterium Acidiphilium rubrum was different from that in the purple bacteria. A weak interaction between Zn-BChl a's suggested the modification in the reaction kinetics.(3) we applied the normal mode analysis to the antenna protein of cyanobacteria, that is, phycobiliproteins, and concluded that the protein structure is essential for stabilizing the electronic state of chromophores that engages in the successive reaction system.(4) We analyzed the excitation relaxation processes of carotenoids that function as an efficient antenna in algae, and found that very fast vibrational relaxationin the S2 state is essential for the antenna function.

分析了光合作用初级过程中的连续反应系统。我们使用的是生物材料，没有构建人工系统。我们得到了以下四个主要结果。(1)我们分析了能量转移过程中，一个新发现的蓝藻Acaryochloris marina是已知的含有叶绿素d。触角叶绿素a的能量水平低于能量陷阱，但在该生物体内存在能量传递的上坡过程，提示了触角系统的一个新概念。我们还发现了一个含有叶绿素d的新物种，这导致了红藻中叶绿素d存在的最终结论。(2)证明了新发现的含Zn-BChl a光合细菌红色嗜酸菌（Acidiphilium rubrum）中的特殊对的空间取向不同于紫色细菌中的特殊对。Zn-BChl a之间的弱相互作用表明反应动力学的修改。(3)我们对蓝藻的触角蛋白，即藻胆蛋白进行了简正模分析，并得出结论，蛋白质结构对于稳定参与连续反应系统的发色团的电子状态是必不可少的。(4)我们分析了类胡萝卜素的激发弛豫过程，发现S2态的非常快的振动弛豫是天线功能所必需的。

项目成果

Mimuro, M., H.Kikuchi: "Antenna Systems and Energy Transfer in Cyanophyta and Rhodophyta"Antenna book(B.R.Green,and W.W.Parson eds.). (in press). (2002)

Mimuro, M., H.Kikuchi：“蓝藻和红藻中的天线系统和能量转移”天线书（B.R.Green 和 W.W.Parson 编辑）。

T. Ishii, K. Uehara, Y. Ozaki, and M. Mimuro: "The effects of pH and ionic strength on the aggregation of bacteriochlorophyll c in aqueous organic media: possibility of two kinds of aggregation forms."Photochem. Photobiol.. 70. 760-765 (1999)

T. Ishii、K. Uehara、Y. Ozaki 和 M. Mimuro：“pH 和离子强度对水性有机介质中细菌叶绿素 c 聚集的影响：两种聚集形式的可能性。”Photochem。

M. Mimuro, T. Ookubo, D. Takahashi, T. Sakawa, S. Akimoto, I. Yamazaki, and H. Miyashita: "Unique fluorescence properties of a cyanobacterium Gloeobacter violaceus PCC 7421: Reasons for absence of the long-wavelength PS I Chl a fluorescence at -196℃."Plan

M. Mimuro、T. Ookubo、D. Takahashi、T. Sakawa、S. Akimoto、I. Yamazaki 和 H. Miyashita：“蓝细菌 Gloeobacter violaceus PCC 7421 的独特荧光特性：缺乏长波长 PS 的原因I Chl a 在-196℃发出荧光。"计划

S. Akimoto, I. Yamazaki, S. Takaichi, and M. Mimuro: "Excitation relaxation dynamics of linear carotenoids"J. Luminescence. 87-89. 797-799 (2000)

S. Akimoto、I. Yamazaki、S. Takaichi 和 M. Mimuro：“线性类胡萝卜素的激发弛豫动力学”J。

S.Satoh et al.,: "Chlorophyll b expressed in cyanobacteria functions as light-harvesting antenna in photosystem I through flexibility of the proteins."Journal of Biological Chemistry. (in press). (2001)

S.Satoh 等人：“蓝藻中表达的叶绿素 b 通过蛋白质的灵活性在光系统 I 中充当光捕获天线。”生物化学杂志。