Circadian gene expression of the circadian clock gene cluster kaiABC in cyanobacteria.

蓝藻中生物钟基因簇kaiABC的昼夜节律基因表达。

• 批准号: 11304055
• 负责人: ISHIURA Masahiro
• 金额: $ 24.29万
• 依托单位: Nagoya University, Center for Gene Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11304055/
• 关键词: Cyanobacteria; Circadian clock; clock gene; kaiABC; Gene expression; Autoregulation; Feedbak regulation; 藍色細菌; kaiABC; SasA; 分子機構; クリプトクローム; フォトリアーゼ; 青色光受容体; 生物発光; ルシフェラーゼ; リアルタイムモニタリング; シアノバクテリア; 始原菌

We analyzed the circadian clock in cyanobacteria, focused on the circadian clock gene cluster kaiABC and its gene products, KaiA, KaiB, and KaiC, and obtained the following results:1 ) KaiC could bind to ATP and GTP, and it was autophosphorylated in the presence of ATP. These activities were necessary to clock oscillation.2 ) We found SasA which bound to KaiC and enhanced the clock oscillation.3 ) We analyzed the photolyase/cryptocrome family in cyanabacteria.4 ) We found two KaiA-binding domains on KaiC.5 ) We developed a promoter-trap method in cyanobacteria.6 ) Pex functioned as a negative regulator for the expression of the kaiA gene.

我们对蓝藻的生物钟进行了分析，重点研究了生物钟基因簇kaiABC及其基因产物KaiA、KaiB和KaiC，得到以下结果：1)KaiC可以结合ATP和GTP，在ATP存在的情况下发生自磷酸化。这些活动是记录振荡所必需的。2)发现SasA与KaiC结合，增强了时钟振荡。3)分析了蓝藻中光解酶/隐花色素家族。4)在KaiC上发现两个kaia结合结构域。5)我们在蓝藻中开发了一种启动子陷阱方法。6) Pex对kaiA基因的表达起负调控作用。

项目成果

Taniguchi Y et al.: "Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC"FEBS Letters. 496. 86-90 (2001)

Taniguchi Y 等人：“蓝藻生物钟蛋白 KaiC 的两个 KaiA 结合域”FEBS Letters。

Iwasaki et al.: "A KaiC-interacting sensory histidine kinase, SasA, necessary to sustain robust circadian oscillation in cyanobacteria"Cell. 101. 223-233 (2000)

Iwasaki 等人：“一种与 KaiC 相互作用的感觉组氨酸激酶，SasA，是维持蓝藻中强大的昼夜节律振荡所必需的”细胞。

Tanuguchi, Y., et al.: "Two-KaiA-binding domains of cyanobacterial circadian clock protein KaiC"FEBS Lett.. 496. 86-90 (2001)

Tanuguchi, Y., et al.：“蓝藻生物钟蛋白 KaiC 的两个 KaiA 结合域”FEBS Lett.. 496. 86-90 (2001)

Iwasaki, H., et al.: "Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria"EMBO. J. 18. 1137-1145 (1999)

Iwasaki, H. 等人：“蓝藻中生物钟蛋白 KaiA、KaiB 和 KaiC 之间的物理相互作用”EMBO。

Nishiwaki,T.: "Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria"Proc.Natl.Acad.Sci.,U.S.A. 97. 495-499 (2000)

Nishiwaki,T.：“时钟蛋白 KaiC 的核苷酸结合和自磷酸化作为蓝细菌的昼夜节律计时过程”Proc.Natl.Acad.Sci.，美国。