Molecular mechanism for circadian clock molecular machinery at the stomic level

生物钟分子机制在原子水平上的分子机制

• 批准号: 15370004
• 负责人: ISHIURA Masahiro
• 金额: $ 9.98万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15370004/
• 关键词: Biological clock; Molecular machinery; Cyanobacteria; Clock proteins; Clock genes; Arabidopsis; Bioluminescence; Real-time monitoring; 藍色細菌; KaiC; 六量体; リン酸化; 相互作用; DNAマイクロアレイ; 発光レポーター遺伝子; X線結晶構造解析

The cyanobacteria clock machinery is composed of three Kai proteins, KaiA, KaiC, and KaiC. We found that KaiC forms hexmers in the presence of ATP and clarified the three-dimensional structure of KaiC hexamer by electron microscopic observation followed by single particle analysis. We determined the stoichiomertic interactions between KaiA and KaiC. We determined the crystal structure of the C-terminal clock oscillator domain of KaiC and identified His270 as a functional residue. Based on the structure, we demonstrated the structure-function relationships of KaiA. We determined the different roles of the two domains, N-terminal and C-terminal domains, of KaiC.

蓝细菌的生物钟由三种Kai蛋白质KaiA、KaiC和KaiC组成。我们发现KaiC在ATP存在下形成六聚体，并通过电子显微镜观察和单粒子分析澄清了KaiC六聚体的三维结构。我们确定了KaiA和KaiC之间的化学计量相互作用。我们确定了KaiC的C-末端时钟振荡器结构域的晶体结构，并将His 270鉴定为功能残基。在此基础上，我们对KaiA的结构与功能关系进行了初步的探讨。我们确定了KaiC的两个结构域，N-末端和C-末端结构域的不同作用。

项目成果

Improvement of the bioluminescent reporter system for real-time monitoring of circadian rhythms in the cyanobacterium Synechocystis sp. Strain PPC6803

改进生物发光报告系统，用于实时监测蓝藻集胞藻的昼夜节律。

• 发表时间: 2005
• 期刊: Genes Genet.Syst 80
• 作者: Kucho;K. et al.
• 通讯作者: K. et al.

Improvement of the bioluminescent reporter system for real-time monitoring of circadian rhythms hi the cyanobacterium Synechocystis sp.strain PCC 6803.

改进生物发光报告系统，实时监测蓝藻集胞藻菌株 PCC 6803 的昼夜节律。

• 发表时间: 2005
• 期刊: Genes Genet.Syst. 80
• 作者: Kucho;K.;Aoki;K.;Itoh;S.;Ishiura;M.
• 通讯作者: M.

Identification of novel clock-regulated genes by cDNA macroarray analysis in Chlamydomonas reihardtii

通过莱氏衣藻 cDNA 宏阵列分析鉴定新型时钟调节基因

• 期刊: Plant Mol.Biol. (印刷中)
• 作者: Kucho;K.;Okamoto;K.;Tabata;S.;Fukuzawa;H.;Ishiura;M.
• 通讯作者: M.

Stoichiometric interactions between cyanobacterial clock proteins KaiA and KaiC

• DOI: 10.1016/j.bbrc.2004.02.034
• 发表时间: 2004-03-26
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Hayashi, F;Ito, H;Ishiura, M
• 通讯作者: Ishiura, M

Identification of novel clock-regulated genes by cDNA macroarray analysis in Chlamydomonas reinhardtii.

通过莱茵衣藻的 cDNA 宏阵列分析鉴定新型时钟调节基因。

• 发表时间: 2005
• 期刊: Plant Mol.Biol. 57
• 作者: Kucho;K.;Okamoto;K.;Tabatu;S.;Fukuzawa;H.;Ishiura;M.
• 通讯作者: M.