"Development of chiral-selective amine acid sensors"

“手性选择性氨基酸传感器的开发”

• 批准号: 11640609
• 负责人: NAGAOKA Tsutomu
• 金额: $ 2.24万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11640609/
• 关键词: Conducting Potymer; Overoxidation; Molecular Recognition; Enantiomer; Amino Acid; Sensor; Templated Potymer; Polypyrrole

Overoxidized polypyrrole films templated with L-glutamate have been utilized for enantiomeric separation of L-and D-glutamic acid. Various important fabrication factors controlling the performance of the overoxidized polypyrrole films have been investigated using fluorescence spectrometry in conjugation with the electrochemical quartz crystal microbalance technique. The measured fluorescence intensity was related to the concentration of glutamate taken up into the films. It was found that L-glutamate was inserted about 30 times as much into the film as D-glutamate. Several key parameters such as applied potential, and pH of amino acid solution were varied so as to achieve the optimum sensor response. The sensor templated with L-glutamic acid also exhibited excellent selectivity over several other L-and D-amino acids. Higher enantioselectivity of overoxidized polypyrrole film than that for previously reported imprinted polymers can be attributed to the potential-induced uptake/release of targeted molecules.

以 L-谷氨酸为模板的过氧化聚吡咯薄膜已用于 L-和 D-谷氨酸的对映体分离。使用荧光光谱法与电化学石英晶体微天平技术相结合，研究了控制过氧化聚吡咯薄膜性能的各种重要制造因素。测量的荧光强度与膜中吸收的谷氨酸浓度有关。结果发现，L-谷氨酸插入薄膜中的量大约是D-谷氨酸的30倍。改变几个关键参数，例如施加的电位和氨基酸溶液的 pH 值，以获得最佳的传感器响应。以 L-谷氨酸为模板的传感器还表现出比其他几种 L-和 D-氨基酸优异的选择性。过氧化聚吡咯薄膜比先前报道的印迹聚合物具有更高的对映选择性，这可归因于潜在诱导的目标分子的摄取/释放。

项目成果

Z.Chen,T.Nagaoka: "Ligand exchange stripping technique for enantio concentration and separation of amino acids"Electroanalysis. 11. 1169-1171 (1999)

Z.Chen，T.Nagaoka：“用于对映体浓缩和氨基酸分离的配体交换剥离技术”电分析。

Z.Chen, Y.Takei, B.A.Deore, and T.Nagaoka: "Enantioselective uptake of amino acid with overoxidized polypyrrole colloid templated with L-lactate"Analyst. 125. 2249-2259 (2000)

Z.Chen、Y.Takei、B.A.Deore 和 T.Nagaoka：“以 L-乳酸为模板的过氧化聚吡咯胶体对氨基酸的对映选择性吸收”分析员。

T.Nagaoka,K.Ogura: "Electroactivity of polypyrrole colloids enhanced by quinone mediation"J.Electrochem.Soc.. 146. 3378-3382 (1999)

T.Nagaoka，K.Ogura：“通过醌介导增强聚吡咯胶体的电活性”J.Electrochem.Soc.. 146. 3378-3382 (1999)

Z.Chen,Y.Takei,B.A.Deore,and T.Nagaoka: "Enantioselective uptake of amino acid with overoxidized polypyrrole colloid templated with L-lactate"Analyst. 125. 2249-2259 (2000)

Z.Chen、Y.Takei、B.A.Deore 和 T.Nagaoka：“以 L-乳酸为模板的过氧化聚吡咯胶体对氨基酸的对映选择性摄取”分析员。

Z.Chen,T.Nagaoka: "Ligand exchange stripping technique for enantio-concentration and separation of amino acies"Electroanalysis. 11. 1169-1171 (1999)

Z.Chen，T.Nagaoka：“用于对映体浓缩和氨基酸分离的配体交换剥离技术”电分析。