DEVELOPMENT OF HIGHLY ACCURATE SENSORS USING MOLECULAR IMPRINTING TECHNIQUE AND THEIR APPLICATIONS TO BIO-RELATED COMPOUNDS

近十年发展起来的分子印迹技术正在成为制备识别目标分子的人工聚合物的有力工具。然而，这种方法在选择性方面仍然存在重要问题，这一直阻碍了它在传感器技术中的使用。为了解决这一问题，我们一直将过氧化技术应用于聚吡咯(PPy)薄膜的制备。在这个项目中，我们利用上述技术和自组装技术开发了高选择性的传感器，并将其应用于生物相关化合物。在这种情况下，胆固醇被印迹在烷硫醇的单分子层上，并将该电极用于测量人体皮肤的胆固醇水平。在9 MHz的Au-EQCM电极上制备了胆汁酸印迹的过氧化聚吡咯膜。该传感器可以接收低至25 NM的胆汁酸发出的信号。

Molecular imprinting techniques, which have developed during the last decade, are becoming a powerful tool for the preparation of artificial polymers that recognize target molecules. However, this approach has still important problems in selectivity, which has been discouraging its use in sensor technology. We have been applying the overoxidation technique for the preparation of polypyrrole (PPy) films in order to solve such a problem. In this project, we have developed highly selective sensors using the above and self-assembly techniques for applications to bio-related compounds.Alkanethiol adsorbed on a Au micro-electrode was used as the base element for this type of sensors. In this case, cholesterol was imprinted in the alkanethiol monolayer, and this electrode was applied to measure the skin cholesterol level of human. An overoxidized polypyrrole film imprinted with bile acid was prepared on a 9-MHz Au-EQCM electrode. The sensor could pick up signals arising from bile acid as low as 25 nM levels.