DEVELOPMENT OF HIGHLY ACCURATE SENSORS USING MOLECULAR IMPRINTING TECHNIQUE AND THEIR APPLICATIONS TO BIO-RELATED COMPOUNDS

利用分子印迹技术开发高精度传感器及其在生物相关化合物中的应用

• 批准号: 15350044
• 负责人: NAGAOKA Tsutomu
• 金额: $ 7.04万
• 依托单位: OSAKA PREFECTURE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15350044/
• 关键词: SAM; MOLECULAR IMPRINT; SENSOR; OVEROXIDIZED POLYPYRROLE; CHOLESTEROL; BILE ACIDS; SKIN; EXTRACTION; MIP; 薄膜; 導電性高分子; アミノ酸; 光学異性体; コロイド

Molecular imprinting techniques, which have developed during the last decade, are becoming a powerful tool for the preparation of artificial polymers that recognize target molecules. However, this approach has still important problems in selectivity, which has been discouraging its use in sensor technology. We have been applying the overoxidation technique for the preparation of polypyrrole (PPy) films in order to solve such a problem. In this project, we have developed highly selective sensors using the above and self-assembly techniques for applications to bio-related compounds.Alkanethiol adsorbed on a Au micro-electrode was used as the base element for this type of sensors. In this case, cholesterol was imprinted in the alkanethiol monolayer, and this electrode was applied to measure the skin cholesterol level of human. An overoxidized polypyrrole film imprinted with bile acid was prepared on a 9-MHz Au-EQCM electrode. The sensor could pick up signals arising from bile acid as low as 25 nM levels.

分子印迹技术是近十年来发展起来的，它正在成为制备识别目标分子的人工聚合物的有力工具。然而，这种方法在选择性上仍然存在重要的问题，这阻碍了它在传感器技术中的应用。为了解决这一问题，我们一直在应用过氧化技术制备聚吡咯（PPy）薄膜。在这个项目中，我们利用上述和自组装技术开发了用于生物相关化合物的高选择性传感器。该传感器采用吸附在金微电极上的烷硫醇作为基础元件。在这种情况下，将胆固醇印迹在烷硫醇单层中，并应用该电极测量人体皮肤胆固醇水平。在9 mhz Au-EQCM电极上制备了胆汁酸印迹过氧化聚吡咯薄膜。该传感器可以接收到低至25纳米水平的胆汁酸产生的信号。

项目成果

化学便覧基礎編改訂5版,5章10節9項「電気化学実験材料」

化学手册基础版修订第5版第5章第10节第9节《电化学实验材料》

• 发表时间: 2003
• 作者: 椎木 弘;長岡 勉
• 通讯作者: 長岡 勉

H.Shiigi, T.Nagaoka: "Fabrication Process and Characterization of a Novel Structural Isomer Sensor : Molecularly Imprinted Overoxidized Polypyrrole Film"Electrochemical and Solid-State Letters. 6. H1-H3 (2003)

H.Shiigi、T.Nagaoka：“新型结构异构体传感器的制造工艺和表征：分子印迹过氧化聚吡咯薄膜”电化学和固态快报。

Selective Determination of dopamine based on a self-assembled monolayer electrode with antifoulding activity on protein adsorption

基于具有蛋白质吸附抗污染活性的自组装单层电极选择性测定多巴胺

• 发表时间: 2004
• 期刊: BUNSEKI KAGAKU 53
• 作者: Z.Chen;T.Nagaoka
• 通讯作者: T.Nagaoka

An Overoxidized Polypyrrole/Dodecylsulfonate Micelle Composite Film for Amperometric Serotonin Sensing

• DOI: 10.1149/1.1566420
• 发表时间: 2003-05
• 期刊: Journal of The Electrochemical Society
• 影响因子: 3.9
• 作者: H. Shiigi;K. Okamura;Daisuke Kijima;B. Deore;U. Sree;T. Nagaoka

椎木 弘, 長岡 勉: "化学便覧基礎編改訂5版,「電気化学実験材料」,5章10節9項"丸善. 946 (2003)

Hiroshi Shiiki、Tsutomu Nagaoka：“化学手册基础版，修订第 5 版，‘电化学实验材料’，第 5 章，第 10 节，第 9 项”Maruzen (2003)。