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Population genetic analysis of Adh expression in Arabidopsis thaliana

拟南芥Adh表达的群体遗传分析

基本信息

批准号：
11640620
负责人：
MIYASHITA Naohiko
金额：
$ 2.3万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

This research project aims to reveal maintenance mechanism of DNA variation in natural populations of the wild plant Arbidopsis thaliana, by investigation relationship between DNA polymorphism and phenotypic variation. Our previous studies have shown two unexpected features of DNA polymorphism in nuclear gene regions of this plant species. One is high level of DNA polymorphism, despite selfing nature of this plant species. One is high level of DNA polymorphism, despite selfing nature of this plant species. The other is the presence of two divergent sequence types (dimorphism). This kind of pattern of DNA polymorphism has not been detected other organisms studied. Obviously, dimorphism causes the level of DNA polymorphism. There are two hypotheses concerning the origin and maintenance mechanism of dimorphism. The first is based on evolutionary history of this plant. In the ancient population of A. thaliana, population division occurred, which caused genetic differentiation between the i … More solated populations. Then, after the period of genetic isolation, fusion of isolated populations took place, resulting m dimorphism in the present population. The second requires natural selection, especially balancing selection, such as overdominance. This kind of natural selection favors coexistence of different alleles, here divergent sequence types. In this investigation, two different approaches were taken. One is to examine if the dimorphism is the general phenomenon in A. thaliana nuclear genes, by extending our effort on other nuclear regions. Newly studied regions were ChiB, PgiC, Atmyb2, 5' upstream region of Adh, Acl5, and Als. In Atmyb2, and 5' upstream region of Adh, dimorphism was not detected, and level of DNA polymorphism was low. But, in the other regions, dimorphism and high level of polymorphism were observed. These results indicates that dimorphism is a characteristic of DNA polymorphism in this plant species, causing the high level of polymorphic variation at the species level. All the results were published in scientific journals, or orally presented in meetings. The second was the investigation of correlation between DNA and phenotypic variations. We focused on Adh, using the Northern and SAGE analyses. Unfortunately, satisfactory results were not obtained. However, this kind of analysis will be important, especially in the era of post-genome project. We would like to perform this kind of research in future, utilizing our experience acquired in this investigation. Less

本研究旨在通过对野生植物拟南芥（Arbidopsis thaliana）自然居群DNA多态性与表型变异关系的研究，揭示其DNA变异的维持机制。我们以前的研究表明，这种植物的核基因区的DNA多态性的两个意想不到的特点。一个是高水平的DNA多态性，尽管这种植物的自交性质。一个是高水平的DNA多态性，尽管这种植物的自交性质。另一种是存在两种不同的序列类型（二态性）。这种DNA多态性模式在其他生物体中尚未发现。显然，二态性导致了DNA多态性的水平。关于二态性的起源和维持机制有两种假说。第一种是基于这种植物的进化历史。在A. thaliana，群体发生了分化，引起了群体间的遗传分化 ...更多信息孤立的人群。在遗传隔离期过后，隔离群体发生融合，导致现在群体的m型二态性。第二个需要自然选择，特别是平衡选择，如超显性。这种自然选择有利于不同等位基因的共存，这里是不同的序列类型。在这项调查中，采取了两种不同的方法。一个是检验二态性是否是A.通过将我们的努力扩展到其他核区域，新研究的区域是ChiB、PgiC、Atmyb 2、Adh的5'上游区域、Acl 5和Als。在Atmyb 2和Adh 5'上游区未检测到二型性，DNA多态性水平较低。但是，在其他地区，二态性和高水平的多态性观察。这些结果表明，二型性是该植物DNA多态性的一个特征，导致了物种水平上的高水平多态性变异。所有结果均发表在科学期刊上，或在会议上口头陈述。二是DNA与表型变异的相关性研究。我们使用北方和SAGE分析，重点关注Adh。不幸的是，没有获得令人满意的结果。然而，这种分析将是重要的，特别是在后基因组计划的时代。我们希望利用我们在这次调查中获得的经验，在未来进行此类研究。少