茶皂素是油茶枯中具有生物活性的重要组分，主要为五环三萜类化合物，为一混合物，近年发现有抑制乙醇吸收的作用,但具体物质基础不明确。我们前期研究发现，茶皂素能与乙醇脱氢酶（ADH）特异性结合。本项目拟将ADH固定在磁性纳米粒子上，借助外加磁场，将茶皂素中与ADH特异结合的组分分离出来；通过分离物的ADH酶活性和色谱学手段，确认影响ADH活性的成分，再对该活性成分的酶学特性进行研究并探讨影响乙醇吸收代谢的机制。内容包括：1.磁性纳米酶粒子和粗茶皂素制备；2.活性组分的磁分离；3.ADH活性组分的酶学特性研究；4.ADH活性组分对肝和胃ADH活性及基因表达的影响、对胃排空和胃液分泌的影响及对胃黏膜和肝组织的影响。研究成果可建立复杂体系中功能组分的快速筛选、富集和分离新模式，可阐明茶皂素中ADH活性成分及对乙醇吸收的影响，可为油茶产业可持续发展和原始创新提供支持。

Tea saponin is a mixture of pentacyclic triterpenes, which is an important bioactive component in camellia seed pomace. It is reported that tea saponin can inhibit the absorption of alcohol in recent years, but the specific material is not clear. Our previous studies found that tea saponin can specifically bind to ethanol dehydrogenase (ADH). In this project we intend to separate the components affecting ADH activity (CAAA) from tea saponin by the immobilized ADH on modified magnetic nanoparticles. Through the determination of ADH activity and chromatographic methods, the compounds in CAAA will be identified, and then the enzymatic characteristics of the compounds will be studied, and the mechanism of the compounds affecting alcohol metabolism will be investigated. The following will be included in the project: 1. Preparation of immobilized enzyme magnetic nanoparticles and crude tea saponin. 2. Magnetic separation of active components. 3. Study on the enzymatic properties of the compounds. 4. Investigation of the influence of the compoumds on liver and stomach ADH activity and gene expression, gastric emptying and gastric juice secretion, and gastric mucosa and liver tissue. The project results can establish a new model for rapid screening, enrichment and separation of functional components in complex systems, clarify the CAAA in tea saponin and their effects on ethanol absorption, and provide support for the sustainable development and original innovation of Camellia oil industry.