Detection and Control of Estrogenic Effect of Drinking Water

饮用水雌激素效应的检测与控制

• 批准号: 11650559
• 负责人: ITOH Sadahiko
• 金额: $ 2.37万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11650559/
• 关键词: Estrogenic effect; Endocrine disrupting chemicals; Environmental hormone; MVLN assay; Disinfection; Chlorination; 2,4-dichlorophenol; 34-ジクロロフェノール; MVLN; エストラジオール; ノニルフェノール; 塩素消毒; フミン物質

It is important to evaluate estrogenic effect of disinfection by-products in drinking water in addition to typical possible endocrine disrupting chemicals. Estrogenic effect was measured by the MVLN assay as an estrogen receptor transcriptional activation assay.The results suggested that the isolation procedure of aquatic humic substances using XAD7HP resin can be recommended to detect estrogenic effect of natural water and its chlorinated drinking water by the MVLN assay. Sample volume necessary to the assay would be 1.2L and required concentration factor would be 100 times. Relative importance of organic substances in Lake Biwa water and chlorinated one concentrated using XAD7HP resin is so large in terms of estrogenic effect. It was also suggested that 4-nonyl phenol and 17 β-estradiol would be important chemicals in investigating estrogenic effect of natural water.Estrogenic effect of Lake Biwa water increased by chlorination. Estrogenic effect of chlorinated water was approximately 2.3 times as strong as that of Lake Biwa water itself.The estrogenic effect of Lake Biwa water was reduced by coagulation and granular activated carbon treatment. The estrogenic effect of treated water however, increased by chlorination. The important result is to find that there is a problem equal to the production of trihalomethanes even in the estrogenic effect of drinking water.

除了典型的可能内分泌破坏化学物质外，评估消毒副产品在饮用水中的雌激素作用很重要。通过MVLN分析作为雌激素受体转录激活测定法测量雌激素作用。结果表明，使用XAD7HP树脂的水生腐殖质物质的隔离程序可以建议检测MVLN测定法的天然水及其氯化饮用水的雌激素作用。测定所需的样品体积为1.2L，所需的浓度因子为100倍。在雌激素作用方面，使用XAD7HP树脂中有机物质在Biwa湖水和氯化的一种浓缩物中的相对重要性是如此之大。还提出，在研究天然水的雌激素作用时，4-氯苯酚和17β-雌二醇将是重要的化学物质。氯化湖水的雌激素作用通过氯化增加。氯化水的雌激素作用约为Biwa水本身的强度2.3倍。通过凝结和颗粒状活性炭处理减少了Biwa水的雌激素作用。然而，经过处理的水的雌激素作用增加了氯化。重要的结果是发现即使在饮用水的雌激素作用中，也存在等于三乙烷的产生问题。

项目成果

Sadahiko Itoh: "Characteristics of Estrogenic Effect of Drinking Water and Its Control"Environmental Engineering Research. 37. 333-344 (2000)

伊藤贞彦：《饮用水的雌激素作用特征及其控制》环境工程研究。

伊藤禎彦: "水道水のエストロゲン様作用の特性と制御性に関する研究"環境工学研究論文集. Vol.37. 333-344 (2000)

伊藤贞彦：“自来水雌激素效应的特性及其可控性研究”环境工程研究杂志第37卷333-344（2000）。