Detection and Control of Estrogenic Effect of Drinking Water

饮用水中消毒副产物的雌激素样效应除了典型的可能的内分泌干扰物外，评价其雌激素样效应具有重要意义。结果表明，XAD 7 HP树脂分离水中腐殖酸类物质的方法可用于MVLN法检测天然水及其氯化饮用水的雌激素效应。测定所需的样品体积为1.2 L，所需的浓缩因子为100倍。从雌激素效应的角度来看，琵琶湖水中的有机物和使用XAD 7 HP树脂浓缩的氯化物的相对重要性是如此之大。4-壬基酚和17 β-雌二醇是研究天然水雌激素效应的重要化学物质。氯化水的雌激素效应约为琵琶湖水本身的2.3倍，混凝和颗粒活性炭处理可降低琵琶湖水的雌激素效应。然而，处理过的水的雌激素效应，氯化增加。重要的结果是发现，即使在饮用水的雌激素效应中，也存在与三卤甲烷的产生相等的问题。

It is important to evaluate estrogenic effect of disinfection by-products in drinking water in addition to typical possible endocrine disrupting chemicals. Estrogenic effect was measured by the MVLN assay as an estrogen receptor transcriptional activation assay.The results suggested that the isolation procedure of aquatic humic substances using XAD7HP resin can be recommended to detect estrogenic effect of natural water and its chlorinated drinking water by the MVLN assay. Sample volume necessary to the assay would be 1.2L and required concentration factor would be 100 times. Relative importance of organic substances in Lake Biwa water and chlorinated one concentrated using XAD7HP resin is so large in terms of estrogenic effect. It was also suggested that 4-nonyl phenol and 17 β-estradiol would be important chemicals in investigating estrogenic effect of natural water.Estrogenic effect of Lake Biwa water increased by chlorination. Estrogenic effect of chlorinated water was approximately 2.3 times as strong as that of Lake Biwa water itself.The estrogenic effect of Lake Biwa water was reduced by coagulation and granular activated carbon treatment. The estrogenic effect of treated water however, increased by chlorination. The important result is to find that there is a problem equal to the production of trihalomethanes even in the estrogenic effect of drinking water.